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Sensors 2010, 10(1), 655-669; doi:10.3390/s100100655

Label-Free Toxin Detection by Means of Time-Resolved Electrochemical Impedance Spectroscopy

School of Environmental and Biological Sciences, Rutgers, the State University of New Jersey, New Brunswick, NJ 08901, USA
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Received: 20 November 2009 / Revised: 7 December 2009 / Accepted: 10 December 2009 / Published: 18 January 2010
(This article belongs to the Section Chemical Sensors)
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Abstract

The real-time detection of trace concentrations of biological toxins requires significant improvement of the detection methods from those reported in the literature. To develop a highly sensitive and selective detection device it is necessary to determine the optimal measuring conditions for the electrochemical sensor in three domains: time, frequency and polarization potential. In this work we utilized a time-resolved electrochemical impedance spectroscopy for the detection of trace concentrations of Staphylococcus enterotoxin B (SEB). An anti-SEB antibody has been attached to the nano-porous aluminum surface using 3-aminopropyltriethoxysilane/glutaraldehyde coupling system. This immobilization method allows fabrication of a highly reproducible and stable sensing device. Using developed immobilization procedure and optimized detection regime, it is possible to determine the presence of SEB at the levels as low as 10 pg/mL in 15 minutes. View Full-Text
Keywords: Staphylococcus enterotoxin B; electrochemical impedance; nano-porous aluminum; antibody immobilization; immunoreaction Staphylococcus enterotoxin B; electrochemical impedance; nano-porous aluminum; antibody immobilization; immunoreaction
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Chai, C.; Takhistov, P. Label-Free Toxin Detection by Means of Time-Resolved Electrochemical Impedance Spectroscopy. Sensors 2010, 10, 655-669.

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