Identification and Functional Divergence Analysis of WOX Gene Family in Paper Mulberry
Abstract
:1. Introduction
2. Results
2.1. Subsection Identification and Structure Analysis of BpWOX Genes
2.2. The Phylogenetic Analysis of BpWOX Family
2.3. Transactivation Activity and Subcellular Localization of BpWOX
2.4. The Tissue Specific Expression Profiles of BpWOX Genes
2.5. The Response of BpWOX Genes to Environmental Stresses
3. Discussion
3.1. WOX Family Underwent Obvious Expansion in the WUS Clade
3.2. Three of 10 BpWOX Proteins Are Typical Transcription Factors
3.3. The Function of WOX Genes in Plant Development
3.4. BpWOXs Showed Divergent Expression under Environmental Stresses
4. Materials and Methods
4.1. Plant Material and Treatments
4.2. Isolation and the Phylogenetic Analysis of BpWOX Gene
4.3. qRT-PCR
4.4. Subcellular Localization of BpWOX Proteins
4.5. Transactivation Activity Assay
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
HOX | Homeobox |
WOX | WUSCHEL-related homeobox |
HD | Homeodomain |
RACE | Rapid amplification of cDNA ends |
qRT-PCR | Quantitative real time PCR |
ORF | Open reading frame |
UTR | Untranslated regions |
aa | Amino acid |
MW | Molecular weight |
NJ | Neighbor-Joining |
3-AT | 3-Aminotriazole |
Cd | Cadmium |
QC | Quiescent center |
SAM | Shoot apical meristem |
PRS | Pressed flower |
GA | Gibberellin |
IAA | Indole-3-acetic acid |
ABA | Abscisic acid |
JA | Jasmonic acid |
SA | Salicylic acid |
ETH | Ethylene |
ERF | Ethylene response factor |
EAR | ERF-associated amphiphilic repression |
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Gene Name | GenBank Accession | gDNA Length (bp) | cDNA Length (bp) | ORF Length (bp) | AA Numbers | MW (Da) | Isoelectric Point (pI) |
---|---|---|---|---|---|---|---|
BpWUS | MF420354 | 2931 | 1275 | 900 | 299 | 32518 | 6.14 |
BpWOX1 | MF420355 | 2861 | 1728 | 1212 | 403 | 45811 | 6.87 |
BpWOX2 | MF420356 | 3241 | 1439 | 1095 | 364 | 41653 | 9.69 |
BpWOX3 | MF420357 | 1925 | 1088 | 723 | 240 | 27653 | 8.7 |
BpWOX4 | MF420358 | 1463 | 1105 | 744 | 247 | 27949 | 9.44 |
BpWOX5 | MF420359 | 927 | 805 | 624 | 207 | 24082 | 7.32 |
BpWOX7 | MF420360 | 3055 | 1119 | 858 | 285 | 31261 | 6.4 |
BpWOX8 | MF420361 | 2756 | 1639 | 1260 | 419 | 45929 | 8.26 |
BpWOX9 | MF420362 | 2920 | 1397 | 816 | 271 | 31020 | 6.23 |
BpWOX10 | MF420363 | 2393 | 1256 | 780 | 259 | 29625 | 5.13 |
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Tang, F.; Chen, N.; Zhao, M.; Wang, Y.; He, R.; Peng, X.; Shen, S. Identification and Functional Divergence Analysis of WOX Gene Family in Paper Mulberry. Int. J. Mol. Sci. 2017, 18, 1782. https://doi.org/10.3390/ijms18081782
Tang F, Chen N, Zhao M, Wang Y, He R, Peng X, Shen S. Identification and Functional Divergence Analysis of WOX Gene Family in Paper Mulberry. International Journal of Molecular Sciences. 2017; 18(8):1782. https://doi.org/10.3390/ijms18081782
Chicago/Turabian StyleTang, Feng, Naizhi Chen, Meiling Zhao, Yucheng Wang, Ruiping He, Xianjun Peng, and Shihua Shen. 2017. "Identification and Functional Divergence Analysis of WOX Gene Family in Paper Mulberry" International Journal of Molecular Sciences 18, no. 8: 1782. https://doi.org/10.3390/ijms18081782