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Int. J. Mol. Sci. 2017, 18(8), 1822; doi:10.3390/ijms18081822

Identification of Reference and Biomarker Proteins in Chlamydomonas reinhardtii Cultured under Different Stress Conditions

Institute of Bioenergy, College of Life Sciences, Hebei Agricultural University, Baoding 071001, Hebei, China
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Received: 25 May 2017 / Revised: 16 August 2017 / Accepted: 17 August 2017 / Published: 22 August 2017
(This article belongs to the Special Issue Selected Papers from the 6th National Plant Protein Research Congress)
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Abstract

Reference proteins and biomarkers are important for the quantitative evaluation of protein abundance. Chlamydomonas reinhardtii was grown under five stress conditions (dark, cold, heat, salt, and glucose supplementation), and the OD750 and total protein contents were evaluated on days 0, 1, 2, 4, and 6 of culture. Antibodies for 20 candidate proteins were generated, and the protein expression patterns were examined by western blotting. Reference protein(s) for each treatment were identified by calculating the Pearson’s correlation coefficient (PCC) between target protein abundance and total protein content. Histone H3, beta tubulin 1 (TUB-1), ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (RBCL), and mitochondrial F1F0 ATP synthase subunit 6 (ATPs-6) were the top reference proteins, because they were expressed stably under multiple stress conditions. The average relative-fold change (ARF) value of each protein was calculated to identify biomarkers. Heat shock protein 90B (HSP90B), flagellar associated protein (FAP127) and ATP synthase CF0 A subunit (ATPs-A) were suitable biomarkers for multiple treatments, while receptor of activated protein kinase C1 (RCK1), biotin carboxylase (BCR1), mitochondrial phosphate carrier protein (MPC1), and rubisco large subunit N-methyltransferase (RMT1) were suitable biomarkers for the dark, cold, heat, and glucose treatments, respectively. View Full-Text
Keywords: Chlamydomonas reinhardtii; abiotic stress; reference protein; biomarker; microalgae; antibody; western blotting Chlamydomonas reinhardtii; abiotic stress; reference protein; biomarker; microalgae; antibody; western blotting
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MDPI and ACS Style

Shi, J.; Huang, T.; Chai, S.; Guo, Y.; Wei, J.; Dou, S.; Li, L.; Liu, G. Identification of Reference and Biomarker Proteins in Chlamydomonas reinhardtii Cultured under Different Stress Conditions. Int. J. Mol. Sci. 2017, 18, 1822.

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