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Int. J. Mol. Sci. 2017, 18(8), 1755; doi:10.3390/ijms18081755

Differential Proteomic Analysis Reveals the Effect of Calcium on Malus baccata Borkh. Leaves under Temperature Stress

1,†
,
2,†
,
1,3,* and 1,3,*
1
College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China
2
Tianjin Facility Agriculture Research Institute, Tianjin 301700, China
3
Key Lab of Fruit Quality Development and Regulation of Liaoning province, Shenyang 110866, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 11 May 2017 / Revised: 8 August 2017 / Accepted: 8 August 2017 / Published: 11 August 2017
(This article belongs to the Special Issue Selected Papers from the 6th National Plant Protein Research Congress)
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Abstract

In the cool apple-producing areas of northern China, air temperature during early spring changes in a rapid and dramatic manner, which affects the growth and development of apple trees at the early stage of the growing season. Previous studies have shown that the treatment of calcium can increase the cold tolerance of Malus baccata Borkh., a widely-used rootstock apple tree in northern China. To better understand the physiological function of calcium in the response of M. baccata to temperature stress, we analyzed the effect of calcium treatment (2% CaCl2) on M. baccata leaves under temperature stress. Physiological analysis showed that temperature stress aggravated membrane lipid peroxidation, reduced chlorophyll content and induced photo-inhibition in leaves, whereas these indicators of stress injuries were alleviated by the application of calcium. An isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomics approach was used in this study. Among the 2114 proteins that were detected in M. baccata leaves, 41, 25, and 34 proteins were differentially regulated by the increasing, decreasing, and changing temperature treatments, respectively. Calcium treatment induced 9 and 15 proteins after increasing and decreasing temperature, respectively, in comparison with non-treated plants. These calcium-responsive proteins were mainly related to catalytic activity, binding, and structural molecule activity. Hierarchical cluster analysis indicated that the changes in abundance of the proteins under increasing temperature and changing temperature treatments were similar, and the changes in protein abundance under decreasing temperature and increasing temperature with calcium treatment were similar. The findings of this study will allow a better understanding of the mechanisms underlying the role of calcium in M. baccata leaves under temperature stress. View Full-Text
Keywords: temperature stress; calcium; iTRAQ; leaves; M. baccata temperature stress; calcium; iTRAQ; leaves; M. baccata
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MDPI and ACS Style

Li, L.; Su, H.; Ma, H.; Lyu, D. Differential Proteomic Analysis Reveals the Effect of Calcium on Malus baccata Borkh. Leaves under Temperature Stress. Int. J. Mol. Sci. 2017, 18, 1755.

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