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Int. J. Mol. Sci. 2017, 18(8), 1782; doi:10.3390/ijms18081782

Identification and Functional Divergence Analysis of WOX Gene Family in Paper Mulberry

1
Key Laboratory of Plant Resources, Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China
2
University of Chinese Academy of Sciences, Beijing 100049, China
*
Authors to whom correspondence should be addressed.
Received: 6 July 2017 / Revised: 2 August 2017 / Accepted: 12 August 2017 / Published: 16 August 2017
(This article belongs to the Special Issue Selected Papers from the 6th National Plant Protein Research Congress)
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Abstract

The WOX (WUSCHEL-related homeobox) is a plant-specific transcription factor involved in plant development and stress response. However, few studies have been reported on the WOX gene in woody plants. In this study, 10 BpWOX genes were isolated from paper mulberry by RACE-PCR and categorized into three clades through phylogenetic analysis, ancient, intermediate and WUS clade. Among them, five members had the transcriptional activity detected by yeast one-hybrid and seven were uniquely localized to the nucleus through green fluorescent protein (GFP) observation. The expression patterns of BpWOX genes in different tissues and under diverse treatments were quantified by the qRT-PCR method. Results showed that BpWUS was expressed in the apical bud, stem and root, BpWOX5 and BpWOX7 functioned only in the root tip, and three BpWOXs regulated leaf development redundantly. BpWOX9 and BpWOX10 were induced by indole-3-acetic acid (IAA) or jasmonic acid (JA), while BpWOX2 was repressed by five phytohormones. Interestingly, most BpWOX genes were responsive to the abiotic stress stimuli of drought, salt, cold, and cadmium (CdCl2). Together, our study revealed that BpWOXs were functionally divergent during paper mulberry development and environmental adaptation, which might be related to their evolutionary relationships. Our work will benefit the systematic understanding of the precise function of WOX in plant development and environmental stress responses. View Full-Text
Keywords: WOX transcription factor; paper mulberry; development; phytohormone; stress response WOX transcription factor; paper mulberry; development; phytohormone; stress response
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Tang, F.; Chen, N.; Zhao, M.; Wang, Y.; He, R.; Peng, X.; Shen, S. Identification and Functional Divergence Analysis of WOX Gene Family in Paper Mulberry. Int. J. Mol. Sci. 2017, 18, 1782.

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