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Int. J. Mol. Sci. 2017, 18(6), 1186; doi:10.3390/ijms18061186

Expression Profiling of Strawberry Allergen Fra a during Fruit Ripening Controlled by Exogenous Auxin

Department of Plant Resource Science, Graduate School of Agricultural Science, Kobe University, Rokko, Kobe, Hyogo 657-8501, Japan
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Author to whom correspondence should be addressed.
Academic Editor: María Serrano
Received: 2 May 2017 / Revised: 25 May 2017 / Accepted: 30 May 2017 / Published: 2 June 2017
(This article belongs to the Special Issue Ripening Control and Induction of the Defence and Antioxidant Systems)
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Abstract

Strawberry fruit contain the allergenic Fra a proteins, members of the pathogenesis-related 10 protein family that causes oral allergic syndrome symptoms. Fra a proteins are involved in the flavonoid biosynthesis pathway, which might be important for color development in fruits. Auxin is an important plant hormone in strawberry fruit that controls fruit fleshiness and ripening. In this study, we treated strawberry fruits with exogenous auxin or auxin inhibitors at pre- and post-harvest stages, and analyzed Fra a transcriptional and translational expression levels during fruit development by real-time PCR and immunoblotting. Pre-harvest treatment with 1-naphthaleneacetic acid (NAA) alone did not affect Fra a expression, but applied in conjunction with achene removal NAA promoted fruit pigmentation and Fra a protein accumulation. The response was developmental stage-specific: Fra a 1 was highly expressed in immature fruit, whereas Fra a 2 was expressed in young to ripe fruit. In post-harvest treatments, auxin did not contribute to Fra a induction. Auxin inhibitors delayed fruit ripening; as a result, they seemed to influence Fra a 1 expression. Thus, Fra a expression was not directly regulated by auxin, but might be associated with the ripening process and/or external factors in a paralog-specific manner. View Full-Text
Keywords: allergen; auxin; auxin inhibitor; Fragaria × ananassa; oral allergic syndrome; pathogenesis-related; phytohormone; post-harvest; pre-harvest; ripening regulation allergen; auxin; auxin inhibitor; Fragaria × ananassa; oral allergic syndrome; pathogenesis-related; phytohormone; post-harvest; pre-harvest; ripening regulation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Ishibashi, M.; Yoshikawa, H.; Uno, Y. Expression Profiling of Strawberry Allergen Fra a during Fruit Ripening Controlled by Exogenous Auxin. Int. J. Mol. Sci. 2017, 18, 1186.

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