Expression Profiling of Strawberry Allergen Fra a during Fruit Ripening Controlled by Exogenous Auxin
AbstractStrawberry fruit contain the allergenic Fra a proteins, members of the pathogenesis-related 10 protein family that causes oral allergic syndrome symptoms. Fra a proteins are involved in the flavonoid biosynthesis pathway, which might be important for color development in fruits. Auxin is an important plant hormone in strawberry fruit that controls fruit fleshiness and ripening. In this study, we treated strawberry fruits with exogenous auxin or auxin inhibitors at pre- and post-harvest stages, and analyzed Fra a transcriptional and translational expression levels during fruit development by real-time PCR and immunoblotting. Pre-harvest treatment with 1-naphthaleneacetic acid (NAA) alone did not affect Fra a expression, but applied in conjunction with achene removal NAA promoted fruit pigmentation and Fra a protein accumulation. The response was developmental stage-specific: Fra a 1 was highly expressed in immature fruit, whereas Fra a 2 was expressed in young to ripe fruit. In post-harvest treatments, auxin did not contribute to Fra a induction. Auxin inhibitors delayed fruit ripening; as a result, they seemed to influence Fra a 1 expression. Thus, Fra a expression was not directly regulated by auxin, but might be associated with the ripening process and/or external factors in a paralog-specific manner. View Full-Text
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Ishibashi, M.; Yoshikawa, H.; Uno, Y. Expression Profiling of Strawberry Allergen Fra a during Fruit Ripening Controlled by Exogenous Auxin. Int. J. Mol. Sci. 2017, 18, 1186.
Ishibashi M, Yoshikawa H, Uno Y. Expression Profiling of Strawberry Allergen Fra a during Fruit Ripening Controlled by Exogenous Auxin. International Journal of Molecular Sciences. 2017; 18(6):1186.Chicago/Turabian Style
Ishibashi, Misaki; Yoshikawa, Hiroki; Uno, Yuichi. 2017. "Expression Profiling of Strawberry Allergen Fra a during Fruit Ripening Controlled by Exogenous Auxin." Int. J. Mol. Sci. 18, no. 6: 1186.