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Int. J. Mol. Sci. 2017, 18(6), 1189; doi:10.3390/ijms18061189

Stability of BDNF in Human Samples Stored Up to 6 Months and Correlations of Serum and EDTA-Plasma Concentrations

1
Department of Neurology, Max Planck Institute for Human Cognitive and Brain Sciences, 04103 Leipzig, Germany
2
Clinic for Psychiatry and Psychotherapy, University of Leipzig, 04103 Leipzig, Germany
3
LIFE—Leipzig Research Center for Civilization Diseases, University of Leipzig, 04103 Leipzig, Germany
4
Department of Endocrinology, University Hospital Leipzig, 04103 Leipzig, Germany
5
Institute of Medical Psychology, Goethe University Frankfurt, 60528 Frankfurt, Germany
6
Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University Hospital Leipzig, 04103 Leipzig, Germany
7
Clinic for Cognitive Neurology, University of Leipzig, 04103 Leipzig, Germany
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Guiting Lin
Received: 30 March 2017 / Revised: 10 May 2017 / Accepted: 30 May 2017 / Published: 3 June 2017
(This article belongs to the Special Issue Brain-Derived Neurotrophic Factor)
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Abstract

Brain-derived neurotrophic factor (BDNF), an important neural growth factor, has gained growing interest in neuroscience, but many influencing physiological and analytical aspects still remain unclear. In this study we assessed the impact of storage time at room temperature, repeated freeze/thaw cycles, and storage at −80 °C up to 6 months on serum and ethylenediaminetetraacetic acid (EDTA)-plasma BDNF. Furthermore, we assessed correlations of serum and plasma BDNF concentrations in two independent sets of samples. Coefficients of variations (CVs) for serum BDNF concentrations were significantly lower than CVs of plasma concentrations (n = 245, p = 0.006). Mean serum and plasma concentrations at all analyzed time points remained within the acceptable change limit of the inter-assay precision as declared by the manufacturer. Serum and plasma BDNF concentrations correlated positively in both sets of samples and at all analyzed time points of the stability assessment (r = 0.455 to rs = 0.596; p < 0.004). In summary, when considering the acceptable change limit, BDNF was stable in serum and in EDTA-plasma up to 6 months. Due to a higher reliability, we suggest favoring serum over EDTA-plasma for future experiments assessing peripheral BDNF concentrations. View Full-Text
Keywords: BDNF; brain-derived neurotrophic factor; serum; EDTA-plasma; stability; inter-assay precision; acceptable change limit BDNF; brain-derived neurotrophic factor; serum; EDTA-plasma; stability; inter-assay precision; acceptable change limit
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Polyakova, M.; Schlögl, H.; Sacher, J.; Schmidt-Kassow, M.; Kaiser, J.; Stumvoll, M.; Kratzsch, J.; Schroeter, M.L. Stability of BDNF in Human Samples Stored Up to 6 Months and Correlations of Serum and EDTA-Plasma Concentrations. Int. J. Mol. Sci. 2017, 18, 1189.

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