Next Article in Journal
Clinical Correlates and Prognostic Value of Plasma Galectin-3 Levels in Degenerative Aortic Stenosis: A Single-Center Prospective Study of Patients Referred for Invasive Treatment
Next Article in Special Issue
Transcriptomics, NF-κB Pathway, and Their Potential Spaceflight-Related Health Consequences
Previous Article in Journal
Magnetic Hyperthermia and Oxidative Damage to DNA of Human Hepatocarcinoma Cells
Previous Article in Special Issue
Genome-Wide Analyses of MicroRNA Profiling in Interleukin-27 Treated Monocyte-Derived Human Dendritic Cells Using Deep Sequencing: A Pilot Study
Article Menu
Issue 5 (May) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2017, 18(5), 936; doi:10.3390/ijms18050936

Pan-Cancer Mutational and Transcriptional Analysis of the Integrator Complex

1
Institute of Genetics and Biophysics “Adriano Buzzati Traverso”, CNR, 80131 Naples, Italy
2
Department of Science and Technology, University of Naples “Parthenope”, 80143 Naples, Italy
3
Department of Environmental, Biological, and Pharmaceutical Sciences and Technologies, University of Campania “Luigi Vanvitelli”, 81100 Caserta, Italy
4
Department of Biochemistry, Biophysics and General Pathology, University of Campania “Luigi Vanvitelli”, Via L. De Crecchio, 80138 Naples, Italy
These two authors contributed equally to this work as co-last authors.
*
Author to whom correspondence should be addressed.
Academic Editor: Sabrina Angelini
Received: 10 April 2017 / Revised: 20 April 2017 / Accepted: 23 April 2017 / Published: 29 April 2017
(This article belongs to the Special Issue Transcriptome Profiling in Human Diseases)
View Full-Text   |   Download PDF [4074 KB, uploaded 29 April 2017]   |  

Abstract

The integrator complex has been recently identified as a key regulator of RNA Polymerase II-mediated transcription, with many functions including the processing of small nuclear RNAs, the pause-release and elongation of polymerase during the transcription of protein coding genes, and the biogenesis of enhancer derived transcripts. Moreover, some of its components also play a role in genome maintenance. Thus, it is reasonable to hypothesize that their functional impairment or altered expression can contribute to malignancies. Indeed, several studies have described the mutations or transcriptional alteration of some Integrator genes in different cancers. Here, to draw a comprehensive pan-cancer picture of the genomic and transcriptomic alterations for the members of the complex, we reanalyzed public data from The Cancer Genome Atlas. Somatic mutations affecting Integrator subunit genes and their transcriptional profiles have been investigated in about 11,000 patients and 31 tumor types. A general heterogeneity in the mutation frequencies was observed, mostly depending on tumor type. Despite the fact that we could not establish them as cancer drivers, INTS7 and INTS8 genes were highly mutated in specific cancers. A transcriptome analysis of paired (normal and tumor) samples revealed that the transcription of INTS7, INTS8, and INTS13 is significantly altered in several cancers. Experimental validation performed on primary tumors confirmed these findings. View Full-Text
Keywords: integrator complex; somatic mutations; transcriptome profiling; human cancers; TCGA data analysis integrator complex; somatic mutations; transcriptome profiling; human cancers; TCGA data analysis
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Federico, A.; Rienzo, M.; Abbondanza, C.; Costa, V.; Ciccodicola, A.; Casamassimi, A. Pan-Cancer Mutational and Transcriptional Analysis of the Integrator Complex. Int. J. Mol. Sci. 2017, 18, 936.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top