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Int. J. Mol. Sci. 2017, 18(5), 939; doi:10.3390/ijms18050939

Magnetic Hyperthermia and Oxidative Damage to DNA of Human Hepatocarcinoma Cells

1
Cancer Risk Factor Branch, Regional Cancer Prevention Laboratory, ISPO-Cancer Research and Prevention Institute, Florence 50139, Italy
2
Nanobiotechnology Department, Colorobbia Consulting-Cericol, Sovigliana, Vinci 50053, Italy
3
Department of Experimental and Clinical Biomedical Sciences, University of Florence, Florence 50139, Italy
4
Department of Pharmaceutical Sciences in the Bouve College of Health Sciences, Barnett Institute, Northeastern University, Boston, MA 02115, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Ashis Basu and Takehiko Nohmi
Received: 1 March 2017 / Revised: 13 April 2017 / Accepted: 23 April 2017 / Published: 29 April 2017
(This article belongs to the Special Issue Chemically-Induced DNA Damage, Mutagenesis, and Cancer)
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Abstract

Nanotechnology is addressing major urgent needs for cancer treatment. We conducted a study to compare the frequency of 3-(2-deoxy-β-d-erythro-pentafuranosyl)pyrimido[1,2-α]purin-10(3H)-one deoxyguanosine (M1dG) and 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) adducts, biomarkers of oxidative stress and/or lipid peroxidation, on human hepatocarcinoma HepG2 cells exposed to increasing levels of Fe3O4-nanoparticles (NPs) versus untreated cells at different lengths of incubations, and in the presence of increasing exposures to an alternating magnetic field (AMF) of 186 kHz using 32P-postlabeling. The levels of oxidative damage tended to increase significantly after ≥24 h of incubations compared to controls. The oxidative DNA damage tended to reach a steady-state after treatment with 60 μg/mL of Fe3O4-NPs. Significant dose–response relationships were observed. A greater adduct production was observed after magnetic hyperthermia, with the highest amounts of oxidative lesions after 40 min exposure to AMF. The effects of magnetic hyperthermia were significantly increased with exposure and incubation times. Most important, the levels of oxidative lesions in AMF exposed NP treated cells were up to 20-fold greater relative to those observed in nonexposed NP treated cells. Generation of oxidative lesions may be a mechanism by which magnetic hyperthermia induces cancer cell death. View Full-Text
Keywords: magnetic therapy; nanotoxicity; M1dG; 8-oxodG; human hepatocarcinoma cells magnetic therapy; nanotoxicity; M1dG; 8-oxodG; human hepatocarcinoma cells
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Cellai, F.; Munnia, A.; Viti, J.; Doumett, S.; Ravagli, C.; Ceni, E.; Mello, T.; Polvani, S.; Giese, R.W.; Baldi, G.; Galli, A.; Peluso, M.E.M. Magnetic Hyperthermia and Oxidative Damage to DNA of Human Hepatocarcinoma Cells. Int. J. Mol. Sci. 2017, 18, 939.

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