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Int. J. Mol. Sci. 2017, 18(5), 1080; doi:10.3390/ijms18051080

Bordetella holmesii: Lipid A Structures and Corresponding Genomic Sequences Comparison in Three Clinical Isolates and the Reference Strain ATCC 51541

1
Institut Pasteur, Unité de Prévention et Thérapies Moléculaires des Maladies Humaines, 25 rue du Dr Roux, 75724 Paris, France
2
Institute for integrative Biology of the Cell (I2BC), Commissariat à l’Energie Atomique (CEA), Centre National de la Recherche Scientifique (CNRS), Université Paris-Sud, Université Paris-Saclay, 91405 Orsay, France
3
LPS-BioSciences, I2BC, Bâtiment 409, Université de Paris-Sud, 91405 Orsay, France
Present address: Dipartimento di Biologia e Biotecnologie “C. Darwin”, Sapienza-Università di Roma, Piazzale Aldo Moro 5, 00185 Roma, Italy.
*
Author to whom correspondence should be addressed.
Academic Editor: Juan M. Tomás
Received: 10 March 2017 / Revised: 5 May 2017 / Accepted: 11 May 2017 / Published: 18 May 2017
(This article belongs to the Special Issue Lipopolysaccharides (LPSs))
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Abstract

Bordetella holmesii can cause invasive infections but can also be isolated from the respiratory tract of patients with whooping-cough like symptoms. For the first time, we describe the lipid A structure of B. holmesii reference strain ATCC 51541 (alias NCTC12912 or CIP104394) and those of three French B. holmesii clinical isolates originating from blood (Bho1) or from respiratory samples (FR4020 and FR4101). They were investigated using chemical analyses, gas chromatography–mass spectrometry (GC–MS), and matrix-assisted laser desorption ionization–mass spectrometry (MALDI–MS). The analyses revealed a common bisphosphorylated β-(1→6)-linked d-glucosamine disaccharide with hydroxytetradecanoic acid in amide linkages. Similar to B. avium, B. hinzii and B. trematum lipids A, the hydroxytetradecanoic acid at the C-2′ position are carrying in secondary linkage a 2-hydroxytetradecanoic acid residue resulting of post-traductional biosynthesis modifications. The three clinical isolates displayed characteristic structural traits compared to the ATCC 51541 reference strain: the lipid A phosphate groups are more or less modified with glucosamine in the isolates and reference strain, but the presence of 10:0(3-OH) is only observed in the isolates. This trait was only described in B. pertussis and B. parapertussis strains, as well as in B. petrii isolates by the past. The genetic bases for most of the key structural elements of lipid A were analyzed and supported the structural data. View Full-Text
Keywords: Bordetellae; Bordetella holmesii; endotoxin; lipid A; structure; mass spectrometry; genomic Bordetellae; Bordetella holmesii; endotoxin; lipid A; structure; mass spectrometry; genomic
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MDPI and ACS Style

Bouchez, V.; AlBitar-Nehmé, S.; Novikov, A.; Guiso, N.; Caroff, M. Bordetella holmesii: Lipid A Structures and Corresponding Genomic Sequences Comparison in Three Clinical Isolates and the Reference Strain ATCC 51541. Int. J. Mol. Sci. 2017, 18, 1080.

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