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Int. J. Mol. Sci. 2017, 18(6), 1163; doi:10.3390/ijms18061163

The Complete Structure of the Core Oligosaccharide from Edwardsiella tarda EIB 202 Lipopolysaccharide

1
Department of Immunochemistry, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, R. Weigla 12, PL-53-114 Wroclaw, Poland
2
Department of Microbiology, University of Barcelona, Diagonal 643, 08071 Barcelona, Spain
3
Department of Biotechnology and Molecular Biology, University of Opole, PL-45-035 Opole, Poland
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: William Chi-shing Cho
Received: 18 February 2017 / Revised: 10 April 2017 / Accepted: 24 May 2017 / Published: 31 May 2017
(This article belongs to the Special Issue Lipopolysaccharides (LPSs))
View Full-Text   |   Download PDF [1505 KB, uploaded 31 May 2017]   |  

Abstract

The chemical structure and genomics of the lipopolysaccharide (LPS) core oligosaccharide of pathogenic Edwardsiella tarda strain EIB 202 were studied for the first time. The complete gene assignment for all LPS core biosynthesis gene functions was acquired. The complete structure of core oligosaccharide was investigated by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy, electrospray ionization mass spectrometry MSn, and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry. The following structure of the undecasaccharide was established: The heterogeneous appearance of the core oligosaccharide structure was due to the partial lack of β-d-Galp and the replacement of α-d-GlcpNAcGly by α-d-GlcpNGly. The glycine location was identified by mass spectrometry. View Full-Text
Keywords: Edwardsiella tarda; core oligosaccharide; MALDI-TOF MS; ESI MSn; NMR; genomic Edwardsiella tarda; core oligosaccharide; MALDI-TOF MS; ESI MSn; NMR; genomic
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Kaszowska, M.; de Mendoza-Barberá, E.; Maciejewska, A.; Merino, S.; Lugowski, C.; Tomás, J.M. The Complete Structure of the Core Oligosaccharide from Edwardsiella tarda EIB 202 Lipopolysaccharide. Int. J. Mol. Sci. 2017, 18, 1163.

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