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Int. J. Mol. Sci. 2017, 18(3), 559; doi:10.3390/ijms18030559

Structural Characterization of Core Region in Erwinia amylovora Lipopolysaccharide

1
Department of Chemical Sciences, University of Naples “Federico II”, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy
2
Division of Bioanalytical Chemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Parkallee 10, D-23845 Borstel, Germany
3
Department of Genética, Microbiología y Estadística, Universidad de Barcelona, Diagonal 643, 08071 Barcelona, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: David Arráez-Román
Received: 2 February 2017 / Revised: 24 February 2017 / Accepted: 28 February 2017 / Published: 4 March 2017
(This article belongs to the Special Issue Lipopolysaccharides (LPSs))
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Abstract

Erwinia amylovora (E. amylovora) is the first bacterial plant pathogen described and demonstrated to cause fire blight, a devastating plant disease affecting a wide range of species including a wide variety of Rosaceae. In this study, we reported the lipopolysaccharide (LPS) core structure from E. amylovora strain CFBP1430, the first one for an E. amylovora highly pathogenic strain. The chemical characterization was performed on the mutants waaL (lacking only the O-antigen LPS with a complete LPS-core), wabH and wabG (outer-LPS core mutants). The LPSs were isolated from dry cells and analyzed by means of chemical and spectroscopic methods. In particular, they were subjected to a mild acid hydrolysis and/or a hydrazinolysis and investigated in detail by one and two dimensional Nuclear Magnetic Resonance (NMR) spectroscopy and ElectroSpray Ionization Fourier Transform-Ion Cyclotron Resonance (ESI FT-ICR) mass spectrometry. View Full-Text
Keywords: lipopolysaccharide; Erwinia amylovora; NMR; ESI FT-ICR; structural determination lipopolysaccharide; Erwinia amylovora; NMR; ESI FT-ICR; structural determination
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MDPI and ACS Style

Casillo, A.; Ziaco, M.; Lindner, B.; Merino, S.; Mendoza-Barberá, E.; Tomás, J.M.; Corsaro, M.M. Structural Characterization of Core Region in Erwinia amylovora Lipopolysaccharide. Int. J. Mol. Sci. 2017, 18, 559.

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