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Int. J. Mol. Sci. 2016, 17(5), 639; doi:10.3390/ijms17050639

Poly(acrylic acid)-regulated Synthesis of Rod-Like Calcium Carbonate Nanoparticles for Inducing the Osteogenic Differentiation of MC3T3-E1 Cells

Bio-X Center, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China
Institute of Natural Sciences, Yonsei University, Seoul 120-749, Korea
College of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou 310018, China
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Academic Editor: Vladimir Sivakov
Received: 20 March 2016 / Revised: 15 April 2016 / Accepted: 22 April 2016 / Published: 6 May 2016
(This article belongs to the Special Issue Inorganic Nanostructures in Biological Systems)
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Calcium carbonate, especially with nanostructure, has been considered as a good candidate material for bone regeneration due to its excellent biodegradability and osteoconductivity. In this study, rod-like calcium carbonate nanoparticles (Rod-CC NPs) with desired water dispersibility were achieved with the regulation of poly (acrylic acid). Characterization results revealed that the Rod-CC NPs had an average length of 240 nm, a width of 90 nm with an average aspect ratio of 2.60 and a negative ζ-potential of −22.25 ± 0.35 mV. The degradation study illustrated the nanoparticles degraded 23% at pH 7.4 and 45% at pH 5.6 in phosphate-buffered saline (PBS) solution within three months. When cultured with MC3T3-E1 cells, the Rod-CC NPs exhibited a positive effect on the proliferation of osteoblast cells. Alkaline phosphatase (ALP) activity assays together with the osteocalcin (OCN) and bone sialoprotein (BSP) expression observations demonstrated the nanoparticles could induce the differentiation of MC3T3-E1 cells. Our study developed well-dispersed rod-like calcium carbonate nanoparticles which have great potential to be used in bone regeneration. View Full-Text
Keywords: calcium carbonate; nanoparticles; rod-like; bone regeneration calcium carbonate; nanoparticles; rod-like; bone regeneration

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Yang, W.; Yao, C.; Cui, Z.; Luo, D.; Lee, I.-S.; Yao, J.; Chen, C.; Kong, X. Poly(acrylic acid)-regulated Synthesis of Rod-Like Calcium Carbonate Nanoparticles for Inducing the Osteogenic Differentiation of MC3T3-E1 Cells. Int. J. Mol. Sci. 2016, 17, 639.

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