Poly(acrylic acid)-regulated Synthesis of Rod-Like Calcium Carbonate Nanoparticles for Inducing the Osteogenic Differentiation of MC3T3-E1 Cells
Abstract
:1. Introduction
2. Results and Discussion
2.1. Preparation and Characterization of Rod-Like Calcium Carbonate Nanoparticles (Rod-CC NPs)
2.2. Cytocompatibility
2.3. Cell Proliferation Assay
2.4. Differentiation of Osteoblast Cells
3. Materials and Methods
3.1. Preparation of Rod-CC NPs
3.2. Characterization of the Rod-CC NPs
3.2.1. Physical–Chemical Characterization
3.2.2. Aqueous Stability of the Rod-CC NPs
3.2.3. In Vitro Rod-CC NPs Degradation
3.3. Cell Culture
3.4. Cytocompatibility Study
3.5. Cell Proliferation Study
3.6. Osteogenic Differentiation Study
3.6.1. Alkaline Phosphatase (ALP) Activity
3.6.2. Osteocalcin and Bone Sialoprotein Production
3.7. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Yang, W.; Yao, C.; Cui, Z.; Luo, D.; Lee, I.-S.; Yao, J.; Chen, C.; Kong, X. Poly(acrylic acid)-regulated Synthesis of Rod-Like Calcium Carbonate Nanoparticles for Inducing the Osteogenic Differentiation of MC3T3-E1 Cells. Int. J. Mol. Sci. 2016, 17, 639. https://doi.org/10.3390/ijms17050639
Yang W, Yao C, Cui Z, Luo D, Lee I-S, Yao J, Chen C, Kong X. Poly(acrylic acid)-regulated Synthesis of Rod-Like Calcium Carbonate Nanoparticles for Inducing the Osteogenic Differentiation of MC3T3-E1 Cells. International Journal of Molecular Sciences. 2016; 17(5):639. https://doi.org/10.3390/ijms17050639
Chicago/Turabian StyleYang, Wei, Chenxue Yao, Zhengyang Cui, Dandan Luo, In-Seop Lee, Juming Yao, Cen Chen, and Xiangdong Kong. 2016. "Poly(acrylic acid)-regulated Synthesis of Rod-Like Calcium Carbonate Nanoparticles for Inducing the Osteogenic Differentiation of MC3T3-E1 Cells" International Journal of Molecular Sciences 17, no. 5: 639. https://doi.org/10.3390/ijms17050639