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Open AccessCommunication
Int. J. Mol. Sci. 2016, 17(7), 1047; doi:10.3390/ijms17071047

In Situ Electron Microscopy of Lactomicroselenium Particles in Probiotic Bacteria

1
Department of Biotechnology and Microbiology, University of Debrecen, Debrecen 4010, Hungary
2
Department of Animal Breeding, University of Debrecen, Debrecen 4010, Hungary
*
Author to whom correspondence should be addressed.
Academic Editor: Vladimir Sivakov
Received: 6 May 2016 / Revised: 22 June 2016 / Accepted: 27 June 2016 / Published: 30 June 2016
(This article belongs to the Special Issue Inorganic Nanostructures in Biological Systems)
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Abstract

Electron microscopy was used to test whether or not (a) in statu nascendi synthesized, and in situ measured, nanoparticle size does not differ significantly from the size of nanoparticles after their purification; and (b) the generation of selenium is detrimental to the bacterial strains that produce them. Elemental nano-sized selenium produced by probiotic latic acid bacteria was used as a lactomicroselenium (lactomicroSel) inhibitor of cell growth in the presence of lactomicroSel, and was followed by time-lapse microscopy. The size of lactomicroSel produced by probiotic bacteria was measured in situ and after isolation and purification. For these measurements the TESLA BS 540 transmission electron microscope was converted from analog (aTEM) to digital processing (dTEM), and further to remote-access internet electron microscopy (iTEM). Lactobacillus acidophilus produced fewer, but larger, lactomicroSel nanoparticles (200–350 nm) than Lactobacillus casei (L. casei), which generated many, smaller lactomicroSel particles (85–200 nm) and grains as a cloudy, less electrodense material. Streptococcus thermophilus cells generated selenoparticles (60–280 nm) in a suicidic manner. The size determined in situ in lactic acid bacteria was significantly lower than those measured by scanning electron microscopy after the isolation of lactomicroSel particles obtained from lactobacilli (100–500 nm), but higher relative to those isolated from Streptococcus thermopilus (50–100 nm). These differences indicate that smaller lactomicroSel particles could be more toxic to the producing bacteria themselves and discrepancies in size could have implications with respect to the applications of selenium nanoparticles as prebiotics. View Full-Text
Keywords: transmission electronmicroscopy; lactomicroSel; nanoparticles; lactobacilli; Streptococcus thermophilus (S. thermophilus) transmission electronmicroscopy; lactomicroSel; nanoparticles; lactobacilli; Streptococcus thermophilus (S. thermophilus)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Nagy, G.; Pinczes, G.; Pinter, G.; Pocsi, I.; Prokisch, J.; Banfalvi, G. In Situ Electron Microscopy of Lactomicroselenium Particles in Probiotic Bacteria. Int. J. Mol. Sci. 2016, 17, 1047.

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