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Open AccessCommunication
Int. J. Mol. Sci. 2016, 17(4), 473; doi:10.3390/ijms17040473

Fluorescence-Lifetime Imaging Microscopy for Visualization of Quantum Dots’ Endocytic Pathway

1
Biophotonics Group of Laser Research Center, Faculty of Physics, Vilnius University, Sauletekio 9, b. 3, Vilnius LT-10222, Lithuania
2
Biomedical Physics Laboratory, National Cancer Institute, P. Baublio 3b, Vilnius LT-08406, Lithuania
3
Department of Chemistry and Bioengineering, Vilnius Gediminas Technical University, Vilnius LT-10223, Lithuania
*
Author to whom correspondence should be addressed.
Academic Editor: Vladimir Sivakov
Received: 23 February 2016 / Revised: 23 March 2016 / Accepted: 23 March 2016 / Published: 30 March 2016
(This article belongs to the Special Issue Inorganic Nanostructures in Biological Systems)
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Abstract

Accumulation of carboxylated polyethylene glycol (PEG) CdSe/ZnSquantum dots (QDs) has been monitored in living fibroblasts using confocal microscopy for fluorescence intensity and fluorescence-lifetime imaging (FLIM). The wide range of mean photoluminescence (PL) lifetime values was observed for the intracellular QDs in different intracellular microenvironment, which revealed structural heterogeneity of endosomes and enabled the distinguishing among endosomes of different maturity. View Full-Text
Keywords: quantum dots; fluorescence-lifetime imaging; endocytosis; intracellular distribution quantum dots; fluorescence-lifetime imaging; endocytosis; intracellular distribution
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Damalakiene, L.; Karabanovas, V.; Bagdonas, S.; Rotomskis, R. Fluorescence-Lifetime Imaging Microscopy for Visualization of Quantum Dots’ Endocytic Pathway. Int. J. Mol. Sci. 2016, 17, 473.

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