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Int. J. Mol. Sci. 2016, 17(11), 1909; doi:10.3390/ijms17111909

miR-30e-5p and miR-15a Synergistically Regulate Fatty Acid Metabolism in Goat Mammary Epithelial Cells via LRP6 and YAP1

1,†
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2,†
,
2,†
,
1,* , 1
,
2
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2,* and 3
1
Shanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China
2
College of Life Sciences, Shenzhen University, Shenzhen 518060, China
3
Mammalian NutriPhysioGenomics, Department of Animal Sciences, Division of Nutritional Sciences, University of Illinois, Urbana, IL 61801, USA
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Kumiko UI-TEI
Received: 31 August 2016 / Revised: 1 November 2016 / Accepted: 9 November 2016 / Published: 16 November 2016
(This article belongs to the Special Issue microRNA Regulation 2017)
View Full-Text   |   Download PDF [2886 KB, uploaded 23 November 2016]   |  

Abstract

MicroRNA (miRNA) regulates the expression of genes and influences a series of biological processes, including fatty acid metabolism. We screened the expression of miRNA in goat mammary glands during peak-lactation and non-lactating (“dry”) periods, and performed an in vitro study with goat mammary epithelial cells (GMEC) prior to sequencing analysis. Results illustrated that miR-30e-5p and miR-15a were highly expressed. Utilizing a luciferase reporter assay and Western blot, low-density lipoprotein receptor-related protein 6 (LRP6) and Yes associated protein 1 (YAP1) genes were demonstrated to be a target of miR-30e-5p and miR-15a in GMEC. Moreover, we demonstrated that the overexpression of miR-30e-5p and miR-15a in GMEC promoted fat metabolism while their knockdown impaired fat metabolism. These findings extend the discovery of a key role of miR-30e-5p and miR-15a in mediating adipocyte differentiation by suggesting a role in promoting milk fat synthesis. In conclusion, our findings indicate that miR-30e-5p, together with miR-15a, represses expression of LRP6 and promotes fat metabolism in GMEC. The data expanded our knowledge on the function of miRNAs in milk fat metabolism and synthesis in ruminant mammary cells. View Full-Text
Keywords: miR-30e-5p; miR-15a; fat metabolism; LRP6; YAP1 miR-30e-5p; miR-15a; fat metabolism; LRP6; YAP1
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Chen, Z.; Qiu, H.; Ma, L.; Luo, J.; Sun, S.; Kang, K.; Gou, D.; Loor, J.J. miR-30e-5p and miR-15a Synergistically Regulate Fatty Acid Metabolism in Goat Mammary Epithelial Cells via LRP6 and YAP1. Int. J. Mol. Sci. 2016, 17, 1909.

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