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Int. J. Mol. Sci. 2016, 17(11), 1799; doi:10.3390/ijms17111799

miR-92a-3p Exerts Various Effects in Glioma and Glioma Stem-Like Cells Specifically Targeting CDH1/β-Catenin and Notch-1/Akt Signaling Pathways

1
State Education Ministry’s Key Laboratory of Developmental Genes and Human Diseases, Southeast University, No. 2 Sipailou Road, Nanjing 210096, China
2
Department of Biochemistry and Molecular Biology, Medical School of Southeast University, No. 87 Dingjiaqiao Road, Nanjing 210009, China
*
Author to whom correspondence should be addressed.
Academic Editor: Andreas Keller
Received: 5 September 2016 / Revised: 18 October 2016 / Accepted: 21 October 2016 / Published: 27 October 2016
(This article belongs to the Special Issue microRNA Regulation 2017)
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Abstract

MicroRNAs (miRNAs) are implicated in the regulation of tumor progression and stemness of cancer stem-like cells. Recently, miR-92a-3p was reported to be up-regulated in human glioma samples. Nevertheless, the precise role of miR-92a-3p in glioma cells and glioma stem-like cells (GSCs) has not been fully elucidated. It is necessary to clarify the function of miR-92a-3p in glioma and GSCs to develop novel therapeutic approaches for glioma patients. In the present study, we applied methyl-thiazolyl-tetrazolium (MTT) assay and Transwell assay to measure the proliferation rate and metastatic potential of glioma cells. Meanwhile, the self-renewal ability of GSCs was detected by tumor sphere formation assay. The results revealed that down-regulation of miR-92a-3p suppressed the glioma cell malignancy in vitro. Moreover, knockdown of miR-92a-3p led to a reduction of tumorgenesis in vivo. Interestingly, we also observed that up-regulation of miR-92a-3p could inhibit the stemness of GSCs. Subsequent mechanistic investigation indicated that cadherin 1 (CDH1)/β-catenin signaling and Notch-1/Akt signaling were the downstream pathways of miR-92a-3p in glioma cells and GSCs, respectively. These results suggest that miR-92a-3p plays different roles in glioma cells and GSCs through regulating different signaling pathways. View Full-Text
Keywords: glioma; glioma stem-like cells; miR-92a-3p; CDH1; Notch-1 glioma; glioma stem-like cells; miR-92a-3p; CDH1; Notch-1
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Song, H.; Zhang, Y.; Liu, N.; Zhao, S.; Kong, Y.; Yuan, L. miR-92a-3p Exerts Various Effects in Glioma and Glioma Stem-Like Cells Specifically Targeting CDH1/β-Catenin and Notch-1/Akt Signaling Pathways. Int. J. Mol. Sci. 2016, 17, 1799.

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