Next Article in Journal
A Multi-Objective Approach for Protein Structure Prediction Based on an Energy Model and Backbone Angle Preferences
Next Article in Special Issue
Hydrogels for Engineering of Perfusable Vascular Networks
Previous Article in Journal
BubR1 Acts as a Promoter in Cellular Motility of Human Oral Squamous Cancer Cells through Regulating MMP-2 and MMP-9
Previous Article in Special Issue
Biomineral/Agarose Composite Gels Enhance Proliferation of Mesenchymal Stem Cells with Osteogenic Capability
Article Menu
Issue 7 (July) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2015, 16(7), 15118-15135; doi:10.3390/ijms160715118

3D-Printed ABS and PLA Scaffolds for Cartilage and Nucleus Pulposus Tissue Regeneration

1
The Orthopedics Research Lab, Department of Surgery, McGill University, Montreal, QC H3G 1A4, Canada
2
McGill Scoliosis & Spine Group, Department of Surgery, McGill University, Montreal, QC H3G 1A4, Canada
3
McGill University Health Centre, Department of Surgery, Montreal General Hospital, Room C10.148.2, 1650 Cedar Ave, Montreal, QC H3G 1A4, Canada
*
Author to whom correspondence should be addressed.
Academic Editor: Bing Yan
Received: 25 April 2015 / Revised: 3 June 2015 / Accepted: 30 June 2015 / Published: 3 July 2015
(This article belongs to the Special Issue Biomaterials for Tissue Engineering)
View Full-Text   |   Download PDF [3172 KB, uploaded 3 July 2015]   |  

Abstract

Painful degeneration of soft tissues accounts for high socioeconomic costs. Tissue engineering aims to provide biomimetics recapitulating native tissues. Biocompatible thermoplastics for 3D printing can generate high-resolution structures resembling tissue extracellular matrix. Large-pore 3D-printed acrylonitrile butadiene styrene (ABS) and polylactic acid (PLA) scaffolds were compared for cell ingrowth, viability, and tissue generation. Primary articular chondrocytes and nucleus pulposus (NP) cells were cultured on ABS and PLA scaffolds for three weeks. Both cell types proliferated well, showed high viability, and produced ample amounts of proteoglycan and collagen type II on both scaffolds. NP generated more matrix than chondrocytes; however, no difference was observed between scaffold types. Mechanical testing revealed sustained scaffold stability. This study demonstrates that chondrocytes and NP cells can proliferate on both ABS and PLA scaffolds printed with a simplistic, inexpensive desktop 3D printer. Moreover, NP cells produced more proteoglycan than chondrocytes, irrespective of thermoplastic type, indicating that cells maintain individual phenotype over the three-week culture period. Future scaffold designs covering larger pore sizes and better mimicking native tissue structure combined with more flexible or resorbable materials may provide implantable constructs with the proper structure, function, and cellularity necessary for potential cartilage and disc tissue repair in vivo. View Full-Text
Keywords: 3D printing; chondrocyte; nucleus pulposus; intervertebral disc; PLA; ABS; tissue engineering 3D printing; chondrocyte; nucleus pulposus; intervertebral disc; PLA; ABS; tissue engineering
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Rosenzweig, D.H.; Carelli, E.; Steffen, T.; Jarzem, P.; Haglund, L. 3D-Printed ABS and PLA Scaffolds for Cartilage and Nucleus Pulposus Tissue Regeneration. Int. J. Mol. Sci. 2015, 16, 15118-15135.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top