Next Article in Journal
Psoralea glandulosa as a Potential Source of Anticancer Agents for Melanoma Treatment
Next Article in Special Issue
High-Throughput Screening for a Moderately Halophilic Phenol-Degrading Strain and Its Salt Tolerance Response
Previous Article in Journal
Pathogenesis of Renal Disease in Systemic Lupus Erythematosus—The Role of Autoantibodies and Lymphocytes Subset Abnormalities
Previous Article in Special Issue
Analysis of Bacterial and Archaeal Communities along a High-Molecular-Weight Polyacrylamide Transportation Pipeline System in an Oil Field
Article Menu
Issue 4 (April) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2015, 16(4), 7932-7943; doi:10.3390/ijms16047932

Promotion of Ni2+ Removal by Masking Toxicity to Sulfate-Reducing Bacteria: Addition of Citrate

1,†
,
1,†
,
1,* , 2
,
1
and
1,*
1
Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences & Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu 610041, China
2
College of Life Science, Sichuan University, Chengdu 610064, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Ji-Dong Gu
Received: 21 November 2014 / Revised: 22 January 2015 / Accepted: 3 April 2015 / Published: 9 April 2015
View Full-Text   |   Download PDF [827 KB, uploaded 9 April 2015]   |  

Abstract

The sulfate-reducing bioprocess is a promising technology for the treatment of heavy metal-containing wastewater. This work was conducted to investigate the possibility of promoting heavy metal removal by the addition of citrate to mask Ni2+ toxicity to sulfate-reducing bacteria (SRB) in batch reactors. SRB growth was completely inhibited in Ni2+-containing medium (1 mM) when lactate served as the sole carbon resource, leading to no sulfate reduction and Ni2+ removal. However, after the addition of citrate, SRB grew well, and sulfate was quickly reduced to sulfide. Simultaneously, the Ni-citrate complex was biodegraded to Ni2+ and acetate. The NiS precipitate was then formed, and Ni2+ was completely removed from the solution. It was suggested that the addition of citrate greatly alleviates Ni2+ toxicity to SRB and improves the removal of Ni2+, which was confirmed by quantitative real-time PCR targeting dissimilatory sulfite reductase (dsrAB) genes. Analysis of the carbon metabolism indicated that lactate instead of acetate served as the electron donor for sulfate reduction. This study offers a potential approach to increase the removal of heavy metals from wastewater in the single stage SRB-based bioprocess. View Full-Text
Keywords: Ni removal; Ni-citrate complex; toxicity masking; lactate; sulfate reduction Ni removal; Ni-citrate complex; toxicity masking; lactate; sulfate reduction
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Qian, J.; Zhu, X.; Tao, Y.; Zhou, Y.; He, X.; Li, D. Promotion of Ni2+ Removal by Masking Toxicity to Sulfate-Reducing Bacteria: Addition of Citrate. Int. J. Mol. Sci. 2015, 16, 7932-7943.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top