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Int. J. Mol. Sci. 2015, 16(4), 7445-7461; doi:10.3390/ijms16047445

Analysis of Bacterial and Archaeal Communities along a High-Molecular-Weight Polyacrylamide Transportation Pipeline System in an Oil Field

1
State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology,130 Meilong Road, Shanghai 200237, China
2
Daqing Oilfield Limited Company, Daqing 163453, China
3
School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, China
*
Author to whom correspondence should be addressed.
Academic Editor: Masato Matsuoka
Received: 29 October 2014 / Revised: 18 March 2015 / Accepted: 19 March 2015 / Published: 2 April 2015
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Abstract

Viscosity loss of high-molecular-weight partially hydrolyzed polyacrylamide (HPAM) solution was observed in a water injection pipeline before being injected into subterranean oil wells. In order to investigate the possible involvement of microorganisms in HPAM viscosity loss, both bacterial and archaeal community compositions of four samples collected from different points of the transportation pipeline were analyzed using PCR-amplification of the 16S rRNA gene and clone library construction method together with the analysis of physicochemical properties of HPAM solution and environmental factors. Further, the relationship between environmental factors and HPAM properties with microorganisms were delineated by canonical correspondence analysis (CCA). Diverse bacterial and archaeal groups were detected in the four samples. The microbial community of initial solution S1 gathered from the make-up tank is similar to solution S2 gathered from the first filter, and that of solution S3 obtained between the first and the second filter is similar to that of solution S4 obtained between the second filter and the injection well. Members of the genus Acinetobacter sp. were detected with high abundance in S3 and S4 in which HPAM viscosity was considerably reduced, suggesting that they likely played a considerable role in HPAM viscosity loss. This study presents information on microbial community diversity in the HPAM transportation pipeline and the possible involvement of microorganisms in HPAM viscosity loss and biodegradation. The results will help to understand the microbial community contribution made to viscosity change and are beneficial for providing information for microbial control in oil fields. View Full-Text
Keywords: microbial community; 16S rRNA gene; CCA; oilfield; HPAM degradation microbial community; 16S rRNA gene; CCA; oilfield; HPAM degradation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Li, C.-Y.; Li, J.-Y.; Mbadinga, S.M.; Liu, J.-F.; Gu, J.-D.; Mu, B.-Z. Analysis of Bacterial and Archaeal Communities along a High-Molecular-Weight Polyacrylamide Transportation Pipeline System in an Oil Field. Int. J. Mol. Sci. 2015, 16, 7445-7461.

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