Transfer RNA Methyltransferases from Thermoplasma acidophilum, a Thermoacidophilic Archaeon
Abstract
:1. Introduction
2. Results and Discussion
2.1. Methylated Nucleosides in T. acidophilum tRNAs
Modification and Position | Candidate Enzyme and Gene | Results from This Study | |
---|---|---|---|
Intiator tRNAMet | |||
m2G26 | Trm1 | Ta0997 | m22G26 and m2G26 formed by Trm1. |
Cm32 | TrmJ? | Ta1010m? | 1 We did not analyze this gene product. |
Cm56 | Trm56 | Ta0931 | Cm56 formed by Trm56. |
m2G57 | ? | 2 This position in the tRNA gene is A57. | |
m1A58 | TrmI | Ta0852? | 3 We could not detect m1A58 formation activity in the recombinant protein. |
Elongator tRNAMet | |||
G*26 | Trm1 | Ta0997 | m22G26 and m2G26 formed by Trm1. |
Cm32 | TrmJ? | Ta1010m? | 1 We did not analyze this gene product. |
Cm56 | Trm56 | Ta0931 | Cm56 formed by Trm56. |
tRNALeuUAG | |||
m22G26 | Trm1 | Ta0997 | m22G26 formed by Trm1. |
m1G37 | Trm5 | Ta0836 | m1G37 formed by Trm5. |
m7G49 | ? | Ta0679? +α? | We could not obtain soluble recombinant protein. |
Cm56 | Trm56 | Ta0931 | Cm56 formed by Trm56. |
m1A58 | TrmI | Ta0852? | 3 We could not detect m1A58 formation activity in the recombinant protein. |
2.2. Transfer RNA Methyltransferase Activities in the Crude Cell Extract
2.3. Modified Nucleosides in Purified tRNAs
2.4. Formation of m1G37 in tRNALeuUAG Transcript by Ta0836 Gene Product
2.5. Ta0997 Gene Product Is a Single Site-Specific Trm1
2.6. T. acidophilum Trm1 Can Modify G26 in Initiator tRNAMet Transcript to m22G26 via m2G26
2.7. T. acidophilum Trm1 Can Methylate the Precursor of Elongator tRNAMet with an Intron
2.8. Ta0931 Gene Product Is Trm56
3. Discussion
4. Experimental Section
4.1. Materials
4.2. Strain, Medium, and Culture
4.3. Preparation of S-30 and S-100 Fractions, and Detection of tRNA Methyltransferase Activities
4.4. Purification of Initiator and Elongator tRNAMet by the Solid-Phase DNA Probe Method
4.5. Nucleoside Analysis
4.6. Selection of Candidate Genes
4.7. Cloning of the Candidate Genes, and Expression of Gene Products
4.7.1. Cloning of Ta0836 (trm5)
4.7.2. Cloning of Ta0997 (trm1)
4.7.3. Cloning of Ta0931 (trm56)
4.7.4. Expression of Gene Products
4.8. Purification of Recombinant Proteins
4.8.1. Purification of Trm5
4.8.2. Purification of Trm1
4.8.3. Purification of Trm56
4.9. Measurement of tRNA Methyltransferase Activities
4.10. Preparation of E. coli RNase P and Removal of 5'-Leader Sequence
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Kawamura, T.; Anraku, R.; Hasegawa, T.; Tomikawa, C.; Hori, H. Transfer RNA Methyltransferases from Thermoplasma acidophilum, a Thermoacidophilic Archaeon. Int. J. Mol. Sci. 2015, 16, 91-113. https://doi.org/10.3390/ijms16010091
Kawamura T, Anraku R, Hasegawa T, Tomikawa C, Hori H. Transfer RNA Methyltransferases from Thermoplasma acidophilum, a Thermoacidophilic Archaeon. International Journal of Molecular Sciences. 2015; 16(1):91-113. https://doi.org/10.3390/ijms16010091
Chicago/Turabian StyleKawamura, Takuya, Ryou Anraku, Takahiro Hasegawa, Chie Tomikawa, and Hiroyuki Hori. 2015. "Transfer RNA Methyltransferases from Thermoplasma acidophilum, a Thermoacidophilic Archaeon" International Journal of Molecular Sciences 16, no. 1: 91-113. https://doi.org/10.3390/ijms16010091