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Int. J. Mol. Sci. 2015, 16(1), 1657-1676; doi:10.3390/ijms16011657

Activation of the Ubiquitin Proteasome Pathway by Silk Fibroin Modified Chitosan Nanoparticles in Hepatic Cancer Cells

1
Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan
2
Translational Research Center, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan
3
Department of Biomedical Engineering, National Yang-Ming University, Taipei 112, Taiwan
4
Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan
5
Department of Nuclear Medicine, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan
6
Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 807, Taiwan
7
Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan
8
Department of Obstetrics and Gynecology, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan
9
School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan
10
Department of Environmental and Occupational Health, National Cheng Kung University, Tainan 701, Taiwan
11
Department of Chemistry, National Sun Yat-sen University, Kaohsiung 804, Taiwan
12
National Sun Yat-sen University–Kaohsiung Medical University Joint Research Center, Kaohsiung 804, Taiwan
13
Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 804, Taiwan
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: David Sheehan
Received: 1 November 2014 / Accepted: 6 January 2015 / Published: 12 January 2015
(This article belongs to the Collection Advances in Proteomic Research)
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Abstract

Silk fibroin (SF) is a protein with bulky hydrophobic domains and can be easily purified as sericin-free silk-based biomaterial. Silk fibroin modified chitosan nanoparticle (SF-CSNP), a biocompatible material, has been widely used as a potential drug delivery system. Our current investigation studied the bio-effects of the SF-CSNP uptake by liver cells. In this experiment, the characterizations of SF-CSNPs were measured by particle size analysis and protein assay. The average size of the SF-CSNP was 311.9 ± 10.7 nm, and the average zeta potential was +13.33 ± 0.3 mV. The SF coating on the SF-CSNP was 6.27 ± 0.17 μg/mL. Moreover, using proteomic approaches, several proteins involved in the ubiquitin proteasome pathway were identified by analysis of differential protein expressions of HepG2 cell uptake the SF-CSNP. Our experimental results have demonstrated that the SF-CSNP may be involved in liver cancer cell survival and proliferation. View Full-Text
Keywords: silk fibroin; chitosan nanoparticle; biomaterial; proteomics; ubiquitin proteasome pathway silk fibroin; chitosan nanoparticle; biomaterial; proteomics; ubiquitin proteasome pathway
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Yang, M.-H.; Chung, T.-W.; Lu, Y.-S.; Chen, Y.-L.; Tsai, W.-C.; Jong, S.-B.; Yuan, S.-S.; Liao, P.-C.; Lin, P.-C.; Tyan, Y.-C. Activation of the Ubiquitin Proteasome Pathway by Silk Fibroin Modified Chitosan Nanoparticles in Hepatic Cancer Cells. Int. J. Mol. Sci. 2015, 16, 1657-1676.

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