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Int. J. Mol. Sci. 2015, 16(1), 1644-1656; doi:10.3390/ijms16011644

Protection of Retina by Mini-αA in NaIO3-Induced Retinal Pigment Epithelium Degeneration Mice

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China
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Author to whom correspondence should be addressed.
Academic Editor: Bing Yan
Received: 11 September 2014 / Accepted: 26 December 2014 / Published: 12 January 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Background: Studies have shown that mini-αA can protect retinal pigment epithelium (RPE) cells from apoptosis. However, no in vivo study concerning the anti-apoptotic function of mini-αA has been conducted yet. Methods: MTT assay, HE staining and TUNEL assay were used to assess levels of cells, and an animal model was established to examine the protective effects of mini-αA against NaIO3-induced RPE cell apoptosis. Western blot analysis and RT-qPCR were performed to explore the possible mechanism of mini-αA’s protective function against NaIO3-induced RPE cell apoptosis. Results: Results from in vivo and animal experiments showed that mini-αA antagonized NaIO3-induced RPE cell apoptosis. Further investigation into how mini-αA provided protection against NaIO3-induced RPE cell apoptosis showed that mini-αA reduced NaIO3-induced RPE cell apoptosis and autophagy. In addition, unfolded protein response was also involved in the protective effects of mini-αA against NaIO3-induced RPE cell apoptosis. Conclusions: mini-αA can antagonize RPE cell apoptosis induced by NaIO3. A possible mechanism is by inhibition of apoptosis by repressing autophagy and endoplasmic reticulum stress. View Full-Text
Keywords: mini-α; NaIO3-induced retinal pigment epithelium; cell apoptosis mini-α; NaIO3-induced retinal pigment epithelium; cell apoptosis
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MDPI and ACS Style

Zhang, J.; Zhao, X.; Cai, Y.; Li, Y.; Yu, X.; Lu, L. Protection of Retina by Mini-αA in NaIO3-Induced Retinal Pigment Epithelium Degeneration Mice. Int. J. Mol. Sci. 2015, 16, 1644-1656.

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