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Int. J. Mol. Sci. 2015, 16(2), 3579-3598; doi:10.3390/ijms16023579

Analysis of Dermal Papilla Cell Interactome Using STRING Database to Profile the ex Vivo Hair Growth Inhibition Effect of a Vinca Alkaloid Drug, Colchicine

1
Institute of Molecular Biology and Department of Life Science, National Chung Cheng University, Chia-yi 621, Taiwan
2
Institute of Stem Cell and Translational Cancer Research, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan
3
Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei 114, Taiwan
4
Department of Ophthalmology, Chia-yi Christian Hospital, Chia-yi 600, Taiwan
*
Authors to whom correspondence should be addressed.
Academic Editor: Bing Yan
Received: 14 November 2014 / Accepted: 3 January 2015 / Published: 5 February 2015
(This article belongs to the Collection Advances in Proteomic Research)
View Full-Text   |   Download PDF [4344 KB, uploaded 5 February 2015]   |  

Abstract

Dermal papillae (DPs) control the formation of hair shafts. In clinical settings, colchicine (CLC) induces patients’ hair shedding. Compared to the control, the ex vivo hair fiber elongation of organ cultured vibrissa hair follicles (HFs) declined significantly after seven days of CLC treatment. The cultured DP cells (DPCs) were used as the experimental model to study the influence of CLC on the protein dynamics of DPs. CLC could alter the morphology and down-regulate the expression of alkaline phosphatase (ALP), the marker of DPC activity, and induce IκBα phosphorylation of DPCs. The proteomic results showed that CLC modulated the expression patterns (fold > 2) of 24 identified proteins, seven down-regulated and 17 up-regulated. Most of these proteins were presumably associated with protein turnover, metabolism, structure and signal transduction. Protein-protein interactions (PPI) among these proteins, established by Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) database, revealed that they participate in protein metabolic process, translation, and energy production. Furthermore, ubiquitin C (UbC) was predicted to be the controlling hub, suggesting the involvement of ubiquitin-proteasome system in modulating the pathogenic effect of CLC on DPC. View Full-Text
Keywords: alopecia; colchicine; dermal papilla; interactome; Protein ANalysis THrough Evolutionary Relationships; Search Tool for the Retrieval of Interacting Genes/Proteins alopecia; colchicine; dermal papilla; interactome; Protein ANalysis THrough Evolutionary Relationships; Search Tool for the Retrieval of Interacting Genes/Proteins
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Hsia, C.-W.; Ho, M.-Y.; Shui, H.-A.; Tsai, C.-B.; Tseng, M.-J. Analysis of Dermal Papilla Cell Interactome Using STRING Database to Profile the ex Vivo Hair Growth Inhibition Effect of a Vinca Alkaloid Drug, Colchicine. Int. J. Mol. Sci. 2015, 16, 3579-3598.

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