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Int. J. Mol. Sci. 2014, 15(4), 5221-5232; doi:10.3390/ijms15045221
Review

Terminal Protection of Small Molecule-Linked DNA for Small Molecule–Protein Interaction Assays

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Received: 25 January 2014 / Revised: 15 March 2014 / Accepted: 17 March 2014 / Published: 25 March 2014
(This article belongs to the collection Proteins and Protein-Ligand Interactions)
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Abstract

Methods for the detection of specific interactions between diverse proteins and various small-molecule ligands are of significant importance in understanding the mechanisms of many critical physiological processes of organisms. The techniques also represent a major avenue to drug screening, molecular diagnostics, and public safety monitoring. Terminal protection assay of small molecule-linked DNA is a demonstrated novel methodology which has exhibited great potential for the development of simple, sensitive, specific and high-throughput methods for the detection of small molecule–protein interactions. Herein, we review the basic principle of terminal protection assay, the development of associated methods, and the signal amplification strategies adopted for performance improving in small molecule–protein interaction assay.
Keywords: protein–ligand interaction; terminal protection; small molecule-linked DNA; signal amplification; nuclease protein–ligand interaction; terminal protection; small molecule-linked DNA; signal amplification; nuclease
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Hu, C.; Wu, Z.; Tang, H.; Tang, L.-J.; Yu, R.-Q.; Jiang, J.-H. Terminal Protection of Small Molecule-Linked DNA for Small Molecule–Protein Interaction Assays. Int. J. Mol. Sci. 2014, 15, 5221-5232.

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