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Int. J. Mol. Sci. 2014, 15(4), 5221-5232; doi:10.3390/ijms15045221

Terminal Protection of Small Molecule-Linked DNA for Small Molecule–Protein Interaction Assays

State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China
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Received: 25 January 2014 / Revised: 15 March 2014 / Accepted: 17 March 2014 / Published: 25 March 2014
(This article belongs to the Collection Proteins and Protein-Ligand Interactions)
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Abstract

Methods for the detection of specific interactions between diverse proteins and various small-molecule ligands are of significant importance in understanding the mechanisms of many critical physiological processes of organisms. The techniques also represent a major avenue to drug screening, molecular diagnostics, and public safety monitoring. Terminal protection assay of small molecule-linked DNA is a demonstrated novel methodology which has exhibited great potential for the development of simple, sensitive, specific and high-throughput methods for the detection of small molecule–protein interactions. Herein, we review the basic principle of terminal protection assay, the development of associated methods, and the signal amplification strategies adopted for performance improving in small molecule–protein interaction assay. View Full-Text
Keywords: protein–ligand interaction; terminal protection; small molecule-linked DNA; signal amplification; nuclease protein–ligand interaction; terminal protection; small molecule-linked DNA; signal amplification; nuclease
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Hu, C.; Wu, Z.; Tang, H.; Tang, L.-J.; Yu, R.-Q.; Jiang, J.-H. Terminal Protection of Small Molecule-Linked DNA for Small Molecule–Protein Interaction Assays. Int. J. Mol. Sci. 2014, 15, 5221-5232.

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