Int. J. Mol. Sci. 2014, 15(4), 6717-6724; doi:10.3390/ijms15046717
Review

Towards Personalized Medicine Mediated by in Vitro Virus-Based Interactome Approaches

email and * email
Received: 2 February 2014; in revised form: 24 March 2014 / Accepted: 9 April 2014 / Published: 21 April 2014
(This article belongs to the collection Proteins and Protein-Ligand Interactions)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: We have developed a simple in vitro virus (IVV) selection system based on cell-free co-translation, using a highly stable and efficient mRNA display method. The IVV system is applicable to the high-throughput and comprehensive analysis of proteins and protein–ligand interactions. Huge amounts of genomic sequence data have been generated over the last decade. The accumulated genetic alterations and the interactome networks identified within cells represent a universal feature of a disease, and knowledge of these aspects can help to determine the optimal therapy for the disease. The concept of the “integrome” has been developed as a means of integrating large amounts of data. We have developed an interactome analysis method aimed at providing individually-targeted health care. We also consider future prospects for this system.
Keywords: cell-free protein synthesis system; in vitro selection; in vitro virus; protein-protein interaction
PDF Full-text Download PDF Full-Text [302 KB, uploaded 19 June 2014 05:37 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Ohashi, H.; Miyamoto-Sato, E. Towards Personalized Medicine Mediated by in Vitro Virus-Based Interactome Approaches. Int. J. Mol. Sci. 2014, 15, 6717-6724.

AMA Style

Ohashi H, Miyamoto-Sato E. Towards Personalized Medicine Mediated by in Vitro Virus-Based Interactome Approaches. International Journal of Molecular Sciences. 2014; 15(4):6717-6724.

Chicago/Turabian Style

Ohashi, Hiroyuki; Miyamoto-Sato, Etsuko. 2014. "Towards Personalized Medicine Mediated by in Vitro Virus-Based Interactome Approaches." Int. J. Mol. Sci. 15, no. 4: 6717-6724.

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert