Int. J. Mol. Sci. 2014, 15(3), 4431-4441; doi:10.3390/ijms15034431
Article

Transplanted Neural Stem Cells Modulate Regulatory T, γδ T Cells and Corresponding Cytokines after Intracerebral Hemorrhage in Rats

1email, 1email, 1email, 1email, 1email, 1email, 2email, 1email, 1email, 2,3email and 1,2,* email
Received: 5 February 2014; in revised form: 27 February 2014 / Accepted: 28 February 2014 / Published: 13 March 2014
(This article belongs to the Special Issue Neurological Injuries’ Monitoring, Tracking and Treatment)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The immune system, particularly T lymphocytes and cytokines, has been implicated in the progression of brain injury after intracerebral hemorrhage (ICH). Although studies have shown that transplanted neural stem cells (NSCs) protect the central nervous system (CNS) from inflammatory damage, their effects on subpopulations of T lymphocytes and their corresponding cytokines are largely unexplored. Here, rats were subjected to ICH and NSCs were intracerebrally injected at 3 h after ICH. The profiles of subpopulations of T cells in the brain and peripheral blood were analyzed by flow cytometry. We found that regulatory T (Treg) cells in the brain and peripheral blood were increased, but γδT cells (gamma delta T cells) were decreased, along with increased anti-inflammatory cytokines (IL-4, IL-10 and TGF-β) and decreased pro-inflammatory cytokines (IL-6, and IFN-γ), compared to the vehicle-treated control. Our data suggest that transplanted NSCs protect brain injury after ICH via modulation of Treg and γδT cell infiltration and anti- and pro-inflammatory cytokine release.
Keywords: ICH; transplantation; NSCs; T lymphocyte subpopulations; immunomodulation
PDF Full-text Download PDF Full-Text [754 KB, uploaded 19 June 2014 05:31 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Gao, L.; Lu, Q.; Huang, L.-J.; Ruan, L.-H.; Yang, J.-J.; Huang, W.-L.; ZhuGe, W.-S.; Zhang, Y.-L.; Fu, B.; Jin, K.-L.; ZhuGe, Q.-C. Transplanted Neural Stem Cells Modulate Regulatory T, γδ T Cells and Corresponding Cytokines after Intracerebral Hemorrhage in Rats. Int. J. Mol. Sci. 2014, 15, 4431-4441.

AMA Style

Gao L, Lu Q, Huang L-J, Ruan L-H, Yang J-J, Huang W-L, ZhuGe W-S, Zhang Y-L, Fu B, Jin K-L, ZhuGe Q-C. Transplanted Neural Stem Cells Modulate Regulatory T, γδ T Cells and Corresponding Cytokines after Intracerebral Hemorrhage in Rats. International Journal of Molecular Sciences. 2014; 15(3):4431-4441.

Chicago/Turabian Style

Gao, Lu; Lu, Qin; Huang, Li-Jie; Ruan, Lin-Hui; Yang, Jian-Jing; Huang, Wei-Long; ZhuGe, Wei-Shan; Zhang, Yong-Liang; Fu, Biao; Jin, Kun-Lin; ZhuGe, Qi-Chuan. 2014. "Transplanted Neural Stem Cells Modulate Regulatory T, γδ T Cells and Corresponding Cytokines after Intracerebral Hemorrhage in Rats." Int. J. Mol. Sci. 15, no. 3: 4431-4441.

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert