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Int. J. Mol. Sci. 2014, 15(12), 21875-21895; doi:10.3390/ijms151221875

Therapeutic Effect of Activated Carbon-Induced Constipation Mice with Lactobacillus fermentum Suo on Treatment

1,†
,
2,†
,
2,†
,
2
,
3
,
1
and
4,*
1
College of Food Science, Southwest University, Chongqing 400715, China
2
Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China
3
Science and Technology Administration, Chinese Academy of Agricultural Sciences, Biejing 100081, China
4
Institute of Qinghai-Tibetan Plateau, Southwest University for Nationalities, Chengdu 610041, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 3 July 2014 / Revised: 26 September 2014 / Accepted: 17 November 2014 / Published: 28 November 2014
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Abstract

The aim of this study was to investigate the effects of Lactobacillus fermentum Suo (LF-Suo) on activated carbon-induced constipation in ICR (Institute of Cancer Research) mice. ICR mice were orally administered with lactic acid bacteria for 9 days. Body weight, diet intake, drinking amount, defecation status, gastrointestinal transit and defecation time, and the serum levels of MTL (motilin), Gas (gastrin), ET (endothelin), SS (somatostatin), AChE (acetylcholinesterase), SP (substance P), VIP (vasoactive intestinal peptide) were used to evaluate the preventive effects of LF-Suo on constipation. Bisacodyl, a laxative drug, was used as a positive control. The normal, control, 100 mg/kg bisacodyl treatment, LB (Lactobacillus bulgaricus)-, LF-Suo (L)- and LF-Suo (H)-treated mice showed the time to the first black stool defecation at 90, 218, 117, 180, 155 and 137 min, respectively. By the oral administration of LB-, LF-Suo (L), LF-Suo (H) or bisacodyl (100 mg/kg), the gastrointestinal transit was reduced to 55.2%, 72.3%, 85.5% and 94.6%, respectively, of the transit in normal mice, respectively. In contrast to the control mice, the serum levels of MTL, Gas, ET, AChE, SP and VIP were significantly increased and the serum levels of SS were reduced in the mice treated with LF-Suo (p < 0.05). By the RT-PCR (reverse transcription–polymerase chain reaction) and western blot assays, LF-Suo increased the c-Kit, SCF (stem cell factor), GDNF (glial cell line-derived neurotrophic factor) and decreased TRPV1 (transient receptor potential vanilloid 1), NOS (nitric oxide synthase) expressions of small intestine tissue in mice. These results demonstrate that lactic acid bacteria has preventive effects on mouse constipation and LF-Suo demonstrated the best functional activity. View Full-Text
Keywords: Lactobacillus fermentum Suo; activated carbon; constipation; bisacodyl; gastrointestinal transit Lactobacillus fermentum Suo; activated carbon; constipation; bisacodyl; gastrointestinal transit
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Suo, H.; Zhao, X.; Qian, Y.; Li, G.; Liu, Z.; Xie, J.; Li, J. Therapeutic Effect of Activated Carbon-Induced Constipation Mice with Lactobacillus fermentum Suo on Treatment. Int. J. Mol. Sci. 2014, 15, 21875-21895.

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