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Int. J. Mol. Sci. 2014, 15(12), 21896-21912; doi:10.3390/ijms151221896

Construction and Characterization of Three Wheat Bacterial Artificial Chromosome Libraries

College of Agricultural Sciences, Nanjing Agricultural University, Nanjing 210095, China
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Received: 21 October 2014 / Revised: 22 November 2014 / Accepted: 24 November 2014 / Published: 28 November 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

We have constructed three bacterial artificial chromosome (BAC) libraries of wheat cultivar Triticum aestivum Wangshuibai, germplasms T. monococcum TA2026 and TA2033. A total of 1,233,792,170,880 and 263,040 clones were picked and arrayed in 384-well plates. On the basis of genome sizes of 16.8 Gb for hexaploid wheat and 5.6 Gb for diploid wheat, the three libraries represented 9.05-, 2.60-, and 3.71-fold coverage of the haploid genomes, respectively. An improved descending pooling system for BAC libraries screening was established. This improved strategy can save 80% of the time and 68% of polymerase chain reaction (PCR) with the same successful rate as the universal 6D pooling strategy. View Full-Text
Keywords: bacterial artificial chromosome; wheat; descending pool bacterial artificial chromosome; wheat; descending pool
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Cao, W.; Fu, B.; Wu, K.; Li, N.; Zhou, Y.; Gao, Z.; Lin, M.; Li, G.; Wu, X.; Ma, Z.; Jia, H. Construction and Characterization of Three Wheat Bacterial Artificial Chromosome Libraries. Int. J. Mol. Sci. 2014, 15, 21896-21912.

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