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Int. J. Mol. Sci. 2014, 15(12), 21644-21659; doi:10.3390/ijms151221644

Proteomic Analysis of Rice Nonhost Resistance to Puccinia striiformis f. sp. tritici Using Two-Dimensional Electrophoresis

State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, No. 3 Taicheng Road, Yangling 712100, China
These authors contributed equally to this work.
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Received: 17 September 2014 / Revised: 9 November 2014 / Accepted: 10 November 2014 / Published: 25 November 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Rice (Oryza sativa L.) is the only widely cultivated gramineous crops that cannot be infected by rust fungi. To decipher the molecular basis of rice nonhost resistance (NHR) to Puccinia striiformis f. sp. tritici (Pst), the causal agent of wheat stripe rust, proteomic analysis was performed using the two-dimensional electrophoresis (2-DE) technique. The expressed proteins from rice leaves 24 and 48 h post inoculation with Pst and from mock-inoculated leaves were identified. Quantitative analysis revealed a total of 27 differentially expressed proteins in response to Pst inoculation. Most of these proteins fall into the category “response to stimulus” and are involved in basic resistance processes, such as glycerol-3-phosphate and hydrogen peroxide signaling. A homologue of wheat leaf rust resistance protein Lr10 was also identified, implicating multiple layers of plant defense are implicated in rice NHR to Pst. These results demonstrate an intrinsic relationship between host and nonhost resistance. Changes in abundance of these proteins, together with their putative functions reveal a comprehensive profile of rice NHR to Pst and provide new insights into plant immunity. View Full-Text
Keywords: nonhost resistance; two-dimensional polyacrylamide gelelectrophoresis (2D-PAGE); proteomics; wheat stripe rust nonhost resistance; two-dimensional polyacrylamide gelelectrophoresis (2D-PAGE); proteomics; wheat stripe rust
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Zhao, J.; Yang, Y.; Kang, Z. Proteomic Analysis of Rice Nonhost Resistance to Puccinia striiformis f. sp. tritici Using Two-Dimensional Electrophoresis. Int. J. Mol. Sci. 2014, 15, 21644-21659.

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