Next Article in Journal
Th17/Treg Cells Imbalance and GITRL Profile in Patients with Hashimoto’s Thyroiditis
Next Article in Special Issue
TGF-β1 Protection against Aβ1–42-Induced Neuroinflammation and Neurodegeneration in Rats
Previous Article in Journal
Proteomic Analysis of Rice Nonhost Resistance to Puccinia striiformis f. sp. tritici Using Two-Dimensional Electrophoresis
Previous Article in Special Issue
Dexamethasone Improves Heat Stroke-Induced Multiorgan Dysfunction and Damage in Rats
Article Menu
Issue 12 (December) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2014, 15(12), 21660-21673; doi:10.3390/ijms151221660

Sterols from Mytilidae Show Anti-Aging and Neuroprotective Effects via Anti-Oxidative Activity

College of Pharmaceutical Sciences, Zhejiang University, Yu Hang Tang Road 866, Hangzhou 310058, China
*
Authors to whom correspondence should be addressed.
Received: 31 July 2014 / Revised: 23 October 2014 / Accepted: 3 November 2014 / Published: 25 November 2014
(This article belongs to the Special Issue Neuroprotective Strategies 2014)
View Full-Text   |   Download PDF [5241 KB, uploaded 25 November 2014]   |  

Abstract

For screening anti-aging samples from marine natural products, K6001 yeast strain was employed as a bioassay system. The active mussel extract was separated to give an active sterol fraction (SF). SF was further purified, and four sterol compounds were obtained. Their structures were determined to be cholesterol (CHOL), brassicasterol, crinosterol, and 24-methylenecholesterol. All compounds showed similar anti-aging activity. To understand the action mechanism involved, anti-oxidative experiments, reactive oxygen species (ROS) assays, and malondialdehyde (MDA) tests were performed on the most abundant compound, CHOL. Results indicated that treatment with CHOL increases the survival rate of yeast under oxidative stress and decreases ROS and MDA levels. In addition, mutations of uth1, skn7, sod1, and sod2, which feature a K6001 background, were employed and the lifespans of the mutations were not affected by CHOL. These results demonstrate that CHOL exerts anti-aging effects via anti-oxidative stress. Based on the connection between neuroprotection and anti-aging, neuroprotective experiments were performed in PC12 cells. Paraquat was used to induce oxidative stress and the results showed that the CHOL and SF protect the PC12 cells from the injury induced by paraquat. In addition, these substance exhibited nerve growth factor (NGF) mimic activities again confirmed their neuroprotective function. View Full-Text
Keywords: neuroprotection; anti-aging; anti-oxidative stress; Mytilidae; sterols; UTH1; SOD; Alzheimer’s disease neuroprotection; anti-aging; anti-oxidative stress; Mytilidae; sterols; UTH1; SOD; Alzheimer’s disease
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Sun, Y.; Lin, Y.; Cao, X.; Xiang, L.; Qi, J. Sterols from Mytilidae Show Anti-Aging and Neuroprotective Effects via Anti-Oxidative Activity. Int. J. Mol. Sci. 2014, 15, 21660-21673.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top