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Int. J. Mol. Sci. 2014, 15(12), 21631-21643; doi:10.3390/ijms151221631

Aberrant Expression of Xist in Aborted Porcine Fetuses Derived from Somatic Cell Nuclear Transfer Embryos

1
Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China
2
Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 14 August 2014 / Revised: 29 October 2014 / Accepted: 10 November 2014 / Published: 25 November 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Cloned pigs generated by somatic cell nuclear transfer (SCNT) show a greater ratio of early abortion during mid-gestation than normal controls. X-linked genes have been demonstrated to be important for the development of cloned embryos. To determine the relationship between the expression of X-linked genes and abortion of cloned porcine fetuses, the expression of X-linked genes were investigated by quantitative real-time polymerase chain reaction (q-PCR) and the methylation status of Xist DMR was performed by bisulfate-specific PCR (BSP). q-PCR analysis indicated that there was aberrant expression of X-linked genes, especially the upregulated expression of Xist in both female and male aborted fetuses compared to control fetuses. Results of BSP suggested that hypomethylation of Xist occurred in aborted fetuses, whether male or female. These results suggest that the abnormal expression of Xist may be associated with the abortion of fetuses derived from somatic cell nuclear transfer embryos. View Full-Text
Keywords: porcine; somatic cell nuclear transfer; DNA methylation; Xist porcine; somatic cell nuclear transfer; DNA methylation; Xist
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Yuan, L.; Wang, A.; Yao, C.; Huang, Y.; Duan, F.; Lv, Q.; Wang, D.; Ouyang, H.; Li, Z.; Lai, L. Aberrant Expression of Xist in Aborted Porcine Fetuses Derived from Somatic Cell Nuclear Transfer Embryos. Int. J. Mol. Sci. 2014, 15, 21631-21643.

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