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Circulating MicroRNAs as Biomarkers of Acute Stroke
Department of Biochemistry and Neuroscience Research Centre, Centre for Translational Medicine, Yong Loo Lin School of Medicine, National University of Singapore, 14 Medical Drive, Singapore 117599, Singapore
Department of Medicine, Faculty of Medicine, University Malaya, Kuala Lumpur 50603, Malaysia
Department of Neurology, Singapore General Hospital and National Neuroscience Institute, Singapore 169608, Singapore
Department of Medicine and Diabetes Centre, Khoo Teck Puat Hospital, 90 Yishun Central, Singapore 768828, Singapore
Department of Biochemistry, Faculty of Medicine, MAHSA University College, Kuala Lumpur 59100, Malaysia
Department of Anatomy and Developmental Biology, School of Biomedical Sciences, Faculty of Medicine, Nursing and Health Sciences, Monash University, Clayton, Victoria 3800, Australia
* Author to whom correspondence should be addressed.
Received: 13 November 2013; in revised form: 20 December 2013 / Accepted: 7 January 2014 / Published: 20 January 2014
Abstract: MicroRNAs have been identified as key regulators of gene expression and thus their potential in disease diagnostics, prognosis and therapy is being actively pursued. Deregulation of microRNAs in cerebral pathogenesis has been reported to a limited extent in both animal models and human. Due to the complexity of the pathology, identifying stroke specific microRNAs has been a challenge. This study shows that microRNA profiles reflect not only the temporal progression of stroke but also the specific etiologies. A panel of 32 microRNAs, which could differentiate stroke etiologies during acute phase was identified and verified using a customized TaqMan Low Density Array (TLDA). Furthermore we also found 5 microRNAs, miR-125b-2*, -27a*, -422a, -488 and -627 to be consistently altered in acute stroke irrespective of age or severity or confounding metabolic complications. Differential expression of these 5 microRNAs was also observed in rat stroke models. Hence, their specificity to the stroke pathology emphasizes the possibility of developing these microRNAs into accurate and useful tools for diagnosis of stroke.
Keywords: microRNAs; cerebral ischemia; diagnosis; brain; blood; microarray
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Sepramaniam, S.; Tan, J.-R.; Tan, K.-S.; DeSilva, D.A.; Tavintharan, S.; Woon, F.-P.; Wang, C.-W.; Yong, F.-L.; Karolina, D.-S.; Kaur, P.; Liu, F.-J.; Lim, K.-Y.; Armugam, A.; Jeyaseelan, K. Circulating MicroRNAs as Biomarkers of Acute Stroke. Int. J. Mol. Sci. 2014, 15, 1418-1432.
Sepramaniam S, Tan J-R, Tan K-S, DeSilva DA, Tavintharan S, Woon F-P, Wang C-W, Yong F-L, Karolina D-S, Kaur P, Liu F-J, Lim K-Y, Armugam A, Jeyaseelan K. Circulating MicroRNAs as Biomarkers of Acute Stroke. International Journal of Molecular Sciences. 2014; 15(1):1418-1432.
Sepramaniam, Sugunavathi; Tan, Jun-Rong; Tan, Kay-Sin; DeSilva, Deidre A.; Tavintharan, Subramaniam; Woon, Fung-Peng; Wang, Chee-Woon; Yong, Fung-Lin; Karolina, Dwi-Setyowati; Kaur, Prameet; Liu, Fu-Jia; Lim, Kai-Ying; Armugam, Arunmozhiarasi; Jeyaseelan, Kandiah. 2014. "Circulating MicroRNAs as Biomarkers of Acute Stroke." Int. J. Mol. Sci. 15, no. 1: 1418-1432.