Int. J. Mol. Sci. 2013, 14(9), 18256-18268; doi:10.3390/ijms140918256
Article

Neuroprotective Effects of α-Tocotrienol on Kainic Acid-Induced Neurotoxicity in Organotypic Hippocampal Slice Cultures

1
, 2
, 3
 and 1,*
Received: 26 July 2013; in revised form: 21 August 2013 / Accepted: 23 August 2013 / Published: 5 September 2013
(This article belongs to the Special Issue Neuroprotective Strategies 2014)
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Abstract: Vitamin E, such as alpha-tocopherol (ATPH) and alpha-tocotrienol (ATTN), is a chain-breaking antioxidant that prevents the chain propagation step during lipid peroxidation. In the present study, we investigated the effects of ATTN on KA-induced neuronal death using organotypic hippocampal slice culture (OHSC) and compared the neuroprotective effects of ATTN and ATPH. After 15 h KA (5 µM) treatment, delayed neuronal death was detected in the CA3 region and reactive oxygen species (ROS) formation and lipid peroxidation were also increased. Both co-treatment and post-treatment of ATPH (100 µM) or ATTN (100 µM) significantly increased the cell survival and reduced the number of TUNEL-positive cells in the CA3 region. Increased dichlorofluorescein (DCF) fluorescence and levels of thiobarbiturate reactive substances (TBARS) were decreased by ATPH and ATTN treatment. These data suggest that ATPH and ATTN treatment have protective effects on KA-induced cell death in OHSC. ATTN treatment tended to be more effective than ATPH treatment, even though there was no significant difference between ATPH and ATTN in co-treatment or post-treatment.
Keywords: kainic acid; organotypic hippocampal slice culture; antioxidant; alpha-tocopherol; alpha-tocotrienol
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Jung, N.Y.; Lee, K.H.; Won, R.; Lee, B.H. Neuroprotective Effects of α-Tocotrienol on Kainic Acid-Induced Neurotoxicity in Organotypic Hippocampal Slice Cultures. Int. J. Mol. Sci. 2013, 14, 18256-18268.

AMA Style

Jung NY, Lee KH, Won R, Lee BH. Neuroprotective Effects of α-Tocotrienol on Kainic Acid-Induced Neurotoxicity in Organotypic Hippocampal Slice Cultures. International Journal of Molecular Sciences. 2013; 14(9):18256-18268.

Chicago/Turabian Style

Jung, Na Y.; Lee, Kyung H.; Won, Ran; Lee, Bae H. 2013. "Neuroprotective Effects of α-Tocotrienol on Kainic Acid-Induced Neurotoxicity in Organotypic Hippocampal Slice Cultures." Int. J. Mol. Sci. 14, no. 9: 18256-18268.


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