Int. J. Mol. Sci. 2013, 14(9), 18239-18255; doi:10.3390/ijms140918239
Article

Bicistronic Gene Transfer Tools for Delivery of miRNAs and Protein Coding Sequences

1,2email, 1,2email and 1,2,* email
Received: 22 July 2013; in revised form: 8 August 2013 / Accepted: 13 August 2013 / Published: 5 September 2013
(This article belongs to the Special Issue Regulation by non-coding RNAs)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: MicroRNAs (miRNAs) are a category of small RNAs that modulate levels of proteins via post-transcriptional inhibition. Currently, a standard strategy to overexpress miRNAs is as mature miRNA duplexes, although this method is cumbersome if multiple miRNAs need to be delivered. Many of these miRNAs are found within introns and processed through the RNA polymerase II pathway. We have designed a vector to exploit this naturally-occurring intronic pathway to deliver the three members of the sensory-specific miR-183 family from an artificial intron. In one version of the vector, the downstream exon encodes the reporter (GFP) while another version encodes a fusion protein created between the transcription factor Atoh1 and the hemaglutinin epitope, to distinguish it from endogenous Atoh1. In vitro analysis shows that the miRNAs contained within the artificial intron are processed and bind to their targets with specificity. The genes downstream are successfully translated into protein and identifiable through immunofluorescence. More importantly, Atoh1 is proven functional through in vitro assays. These results suggest that this cassette allows expression of miRNAs and proteins simultaneously, which provides the opportunity for joint delivery of specific translational repressors (miRNA) and possibly transcriptional activators (transcription factors). This ability is attractive for future gene therapy use.
Keywords: miRNAs; gene therapy; miR-183 family
PDF Full-text Download PDF Full-Text [2746 KB, uploaded 19 June 2014 05:07 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Stoller, M.L.; Chang, H.C.; Fekete, D.M. Bicistronic Gene Transfer Tools for Delivery of miRNAs and Protein Coding Sequences. Int. J. Mol. Sci. 2013, 14, 18239-18255.

AMA Style

Stoller ML, Chang HC, Fekete DM. Bicistronic Gene Transfer Tools for Delivery of miRNAs and Protein Coding Sequences. International Journal of Molecular Sciences. 2013; 14(9):18239-18255.

Chicago/Turabian Style

Stoller, Michelle L.; Chang, Henry C.; Fekete, Donna M. 2013. "Bicistronic Gene Transfer Tools for Delivery of miRNAs and Protein Coding Sequences." Int. J. Mol. Sci. 14, no. 9: 18239-18255.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert