Abstract: MicroRNA-143 (miR-143) was found to be downregulated in allergic rhinitis, and bioinformatics analysis predicted that IL-13Rα1 was a target gene of miR-143. To understand the molecular mechanisms of miR-143 involved in the pathogenesis of allergic inflammation, recombinant miR-143 plasmid vectors were constructed, and human mast cell-1(HMC-1) cells which play a central role in the allergic response were used for study. The plasmids were transfected into HMC-1 cells using a lentiviral vector. Expression of IL-13Rα1 mRNA was then detected by reverse transcriptase polymerase chain reaction (RT-PCR) and Western Blotting. The miR-143 lentiviral vector was successfully stably transfected in HMC-1 cells for target gene expression. Compared to the control, the target gene IL-13Rα1 was less expressed in HMC-1 transfected with miR-143 as determined by RT-PCR and Western Blotting (p < 0.05); this difference in expression was statistically significant and the inhibition efficiency was 71%. It indicates that miR-143 directly targets IL-13Rα1 and suppresses IL-13Rα1 expression in HMC-1 cells. Therefore, miR-143 may be associated with allergic reaction in human mast cells.
Keywords: microRNA-143; IL-13Rα1; mast cell; lentiviral vector; allergy
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Yu, S.; Zhang, R.; Zhu, C.; Cheng, J.; Wang, H.; Wu, J. MicroRNA-143 Downregulates Interleukin-13 Receptor Alpha1 in Human Mast Cells. Int. J. Mol. Sci. 2013, 14, 16958-16969.
Yu S, Zhang R, Zhu C, Cheng J, Wang H, Wu J. MicroRNA-143 Downregulates Interleukin-13 Receptor Alpha1 in Human Mast Cells. International Journal of Molecular Sciences. 2013; 14(8):16958-16969.
Yu, Shaoqing; Zhang, Ruxin; Zhu, Chunshen; Cheng, Jianqiu; Wang, Hong; Wu, Jing. 2013. "MicroRNA-143 Downregulates Interleukin-13 Receptor Alpha1 in Human Mast Cells." Int. J. Mol. Sci. 14, no. 8: 16958-16969.