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Int. J. Mol. Sci. 2013, 14(5), 9604-9617; doi:10.3390/ijms14059604
Article

Isolation of Multipotent Nestin-Expressing Stem Cells Derived from the Epidermis of Elderly Humans and TAT-VHL Peptide-Mediated Neuronal Differentiation of These Cells

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Received: 7 March 2013; in revised form: 17 April 2013 / Accepted: 23 April 2013 / Published: 3 May 2013
(This article belongs to the Special Issue Molecular Research of Epidermal Stem Cells)
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Abstract: A specialized population of cells residing in the hair follicle is quiescent but shows pluripotency for differentiating into epithelial-mesenchymal lineage cells. Therefore, such cells are hoped to be useful as implantable donor cells for regenerative therapy. Recently, it was reported that intracellular delivery of TAT-VHL peptide induces neuronal differentiation of skin-derived precursors. In the present study, we successfully isolated multipotent stem cells derived from the epidermis of elderly humans, characterized these cells as being capable of sphere formation and strong expression of nestin, fibronectin, and CD34 but not of keratin 15, and identified the niche of these cells as being the outer root sheath of the hair follicles. In addition, we showed that TAT-VHL peptide induced their neuronal differentiation in vitro, and confirmed by fluorescence immunohistochemistry the neuronal differentiation of such peptide-treated cells implanted into rodent brains. These multipotent nestin-expressing stem cells derived from human epidermis are easily accessible and should be useful as donor cells for neuronal regenerative cell therapy.
Keywords: hair follicle; multipotent stem cells; nestin; neuronal differentiation hair follicle; multipotent stem cells; nestin; neuronal differentiation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Kanno, H.; Kubo, A.; Yoshizumi, T.; Mikami, T.; Maegawa, J. Isolation of Multipotent Nestin-Expressing Stem Cells Derived from the Epidermis of Elderly Humans and TAT-VHL Peptide-Mediated Neuronal Differentiation of These Cells. Int. J. Mol. Sci. 2013, 14, 9604-9617.

AMA Style

Kanno H, Kubo A, Yoshizumi T, Mikami T, Maegawa J. Isolation of Multipotent Nestin-Expressing Stem Cells Derived from the Epidermis of Elderly Humans and TAT-VHL Peptide-Mediated Neuronal Differentiation of These Cells. International Journal of Molecular Sciences. 2013; 14(5):9604-9617.

Chicago/Turabian Style

Kanno, Hiroshi; Kubo, Atsuhiko; Yoshizumi, Tetsuya; Mikami, Taro; Maegawa, Jiro. 2013. "Isolation of Multipotent Nestin-Expressing Stem Cells Derived from the Epidermis of Elderly Humans and TAT-VHL Peptide-Mediated Neuronal Differentiation of These Cells." Int. J. Mol. Sci. 14, no. 5: 9604-9617.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert