Next Article in Journal
Chebulagic Acid, a Hydrolyzable Tannin, Exhibited Antiviral Activity in Vitro and in Vivo against Human Enterovirus 71
Next Article in Special Issue
Epidermal Development in Mammals: Key Regulators, Signals from Beneath, and Stem Cells
Previous Article in Journal
Where Do They Come from and Where Do They Go: Candidates for Regulating Extracellular Vesicle Formation in Fungi
Previous Article in Special Issue
Irradiated Human Dermal Fibroblasts Are as Efficient as Mouse Fibroblasts as a Feeder Layer to Improve Human Epidermal Cell Culture Lifespan
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2013, 14(5), 9604-9617; doi:10.3390/ijms14059604

Isolation of Multipotent Nestin-Expressing Stem Cells Derived from the Epidermis of Elderly Humans and TAT-VHL Peptide-Mediated Neuronal Differentiation of These Cells

1
Department of Neurosurgery, Yokohama City University, Yokohama 236-0004, Japan
2
Department of Plastic Surgery, Yokohama City University, Yokohama 236-0004, Japan
*
Author to whom correspondence should be addressed.
Received: 7 March 2013 / Revised: 17 April 2013 / Accepted: 23 April 2013 / Published: 3 May 2013
(This article belongs to the Special Issue Molecular Research of Epidermal Stem Cells)
View Full-Text   |   Download PDF [2693 KB, uploaded 19 June 2014]   |  

Abstract

A specialized population of cells residing in the hair follicle is quiescent but shows pluripotency for differentiating into epithelial-mesenchymal lineage cells. Therefore, such cells are hoped to be useful as implantable donor cells for regenerative therapy. Recently, it was reported that intracellular delivery of TAT-VHL peptide induces neuronal differentiation of skin-derived precursors. In the present study, we successfully isolated multipotent stem cells derived from the epidermis of elderly humans, characterized these cells as being capable of sphere formation and strong expression of nestin, fibronectin, and CD34 but not of keratin 15, and identified the niche of these cells as being the outer root sheath of the hair follicles. In addition, we showed that TAT-VHL peptide induced their neuronal differentiation in vitro, and confirmed by fluorescence immunohistochemistry the neuronal differentiation of such peptide-treated cells implanted into rodent brains. These multipotent nestin-expressing stem cells derived from human epidermis are easily accessible and should be useful as donor cells for neuronal regenerative cell therapy.
Keywords: hair follicle; multipotent stem cells; nestin; neuronal differentiation hair follicle; multipotent stem cells; nestin; neuronal differentiation
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Kanno, H.; Kubo, A.; Yoshizumi, T.; Mikami, T.; Maegawa, J. Isolation of Multipotent Nestin-Expressing Stem Cells Derived from the Epidermis of Elderly Humans and TAT-VHL Peptide-Mediated Neuronal Differentiation of These Cells. Int. J. Mol. Sci. 2013, 14, 9604-9617.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top