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Transcriptional Profiling of Swine Lung Tissue after Experimental Infection with Actinobacillus pleuropneumoniae
College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, Sichuan, China
Laboratory of Animal Disease and Human Health, Sichuan Agricultural University, Ya'an 625014, Sichuan, China
College of Animal Science and Technology, Sichuan Agricultural University, Ya'an 625014, Sichuan, China
* Author to whom correspondence should be addressed.
Received: 1 April 2013; in revised form: 9 May 2013 / Accepted: 10 May 2013 / Published: 21 May 2013
Abstract: Porcine pleuropneumonia is a highly contagious respiratory disease that causes great economic losses worldwide. In this study, we aimed to explore the underlying relationship between infection and injury by investigation of the whole porcine genome expression profiles of swine lung tissues post-inoculated with experimentally Actinobacillus pleuropneumoniae. Expression profiling experiments of the control group and the treatment group were conducted using a commercially available Agilent Porcine Genechip including 43,603 probe sets. Microarray analysis was conducted on profiles of lung from challenged versus non-challenged swine. We found 11,929 transcripts, identified as differentially expressed at the p ≤0.01 level. There were 1188 genes annotated as swine genes in the GenBank Data Base. GO term analysis identified a total of 89 biological process categories, 82 cellular components and 182 molecular functions that were significantly affected, and at least 27 biological process categories that were related to the host immune response. Gene set enrichment analysis identified 13 pathways that were significantly associated with host response. Many proinflammatory-inflammatory cytokines were activated and involved in the regulation of the host defense response at the site of inflammation; while the cytokines involved in regulation of the host immune response were suppressed. All changes of genes and pathways of induced or repressed expression not only led to a decrease in antigenic peptides presented to T lymphocytes by APCs via the MHC and alleviated immune response injury induced by infection, but also stimulated stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocyte, and promote neutrophils and macrophages to phagocytose bacterial and foreign antigen at the site of inflammation. The defense function of swine infection with Actinobacillus pleuropneumoniae was improved, while its immune function was decreased.
Keywords: porcine pleuropneumonia; infection; injury; Actinobacillus pleuropneumoniae; agilent porcine genechip; microarray analyses; cytokine; host defense response
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MDPI and ACS Style
Zuo, Z.; Cui, H.; Li, M.; Peng, X.; Zhu, L.; Zhang, M.; Ma, J.; Xu, Z.; Gan, M.; Deng, J.; Li, X.; Fang, J. Transcriptional Profiling of Swine Lung Tissue after Experimental Infection with Actinobacillus pleuropneumoniae. Int. J. Mol. Sci. 2013, 14, 10626-10660.
Zuo Z, Cui H, Li M, Peng X, Zhu L, Zhang M, Ma J, Xu Z, Gan M, Deng J, Li X, Fang J. Transcriptional Profiling of Swine Lung Tissue after Experimental Infection with Actinobacillus pleuropneumoniae. International Journal of Molecular Sciences. 2013; 14(5):10626-10660.
Zuo, Zhicai; Cui, Hengmin; Li, Mingzhou; Peng, Xi; Zhu, Ling; Zhang, Ming; Ma, Jideng; Xu, Zhiwen; Gan, Meng; Deng, Junliang; Li, Xuewei; Fang, Jing. 2013. "Transcriptional Profiling of Swine Lung Tissue after Experimental Infection with Actinobacillus pleuropneumoniae." Int. J. Mol. Sci. 14, no. 5: 10626-10660.