Int. J. Mol. Sci. 2013, 14(10), 20809-20819; doi:10.3390/ijms141020809
Article

Ultrastructural Analysis of Nanogold-Labeled Cell Surface Microvilli in Liquid by Atmospheric Scanning Electron Microscopy and Their Relevance in Cell Adhesion

Received: 6 September 2013; in revised form: 6 October 2013 / Accepted: 7 October 2013 / Published: 16 October 2013
(This article belongs to the Special Issue Frontiers of Micro-Spectroscopy in Biological Applications)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The adhesion of leukocytes circulating in the blood to vascular endothelium is critical for their trafficking in the vasculature, and CD44 is an important cell surface receptor for rolling adhesion. In this study, we demonstrate the correlative observation of CD44 distribution at the lymphocyte cell surface in liquid by fluorescence optical microscopy and immuno-electron microscopy using an atmospheric scanning electron microscope (ASEM). The ultrastructure of the cell surface was clearly imaged by ASEM using positively charged Nanogold particles. ASEM analysis demonstrated microvilli projections around the cell surface and the localization of CD44 on the microvilli. Treatment of cells with cytochalasin D resulted in a loss of the microvilli projections and concomitantly abrogated CD44-mediated adhesion to its ligand hyaluronan. These results suggest the functional relevance of microvilli in CD44-mediated rolling adhesion under shear flow.
Keywords: cell adhesion; cell surface; cytoskeleton; microvilli; immuno-electron microscopy; SEM; correlative microscopy; correlative light and electron microscopy (CLEM); ASEM
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MDPI and ACS Style

Murai, T.; Sato, M.; Nishiyama, H.; Suga, M.; Sato, C. Ultrastructural Analysis of Nanogold-Labeled Cell Surface Microvilli in Liquid by Atmospheric Scanning Electron Microscopy and Their Relevance in Cell Adhesion. Int. J. Mol. Sci. 2013, 14, 20809-20819.

AMA Style

Murai T, Sato M, Nishiyama H, Suga M, Sato C. Ultrastructural Analysis of Nanogold-Labeled Cell Surface Microvilli in Liquid by Atmospheric Scanning Electron Microscopy and Their Relevance in Cell Adhesion. International Journal of Molecular Sciences. 2013; 14(10):20809-20819.

Chicago/Turabian Style

Murai, Toshiyuki; Sato, Mari; Nishiyama, Hidetoshi; Suga, Mitsuo; Sato, Chikara. 2013. "Ultrastructural Analysis of Nanogold-Labeled Cell Surface Microvilli in Liquid by Atmospheric Scanning Electron Microscopy and Their Relevance in Cell Adhesion." Int. J. Mol. Sci. 14, no. 10: 20809-20819.

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