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Inhibition of Oxidative Stress by Low-Molecular-Weight Polysaccharides with Various Functional Groups in Skin Fibroblasts
Department of Food Science, National Taiwan Ocean University, 2 Pei-Ning Road, Keelung 20224, Taiwan
Department of Food and Beverage Management, Yuanpei University, 306, Yuanpei Street, Hsinchu 30015, Taiwan
Cellular Stress and Ageing Program, Bionanoscience and Bio-Imaging Program, Bio & Nano-Solutions, Helmutstr. 3A, Düsseldorf 40472, Germany
* Authors to whom correspondence should be addressed.
Received: 9 July 2013; in revised form: 28 August 2013 / Accepted: 4 September 2013 / Published: 25 September 2013
Abstract: The aim of this study was to evaluate the in cellulo inhibition of hydrogen-peroxide-induced oxidative stress in skin fibroblasts using different low-molecular-weight polysaccharides (LMPS) prepared from agar (LMAG), chitosan (LMCH) and starch (LMST), which contain various different functional groups (i.e., sulfate, amine, and hydroxyl groups). The following parameters were evaluated: cell viability, intracellular oxidant production, lipid peroxidation, and DNA damage. Trolox was used as a positive control in order to allow comparison of the antioxidant efficacies of the various LMPS. The experimentally determined attenuation of oxidative stress by LMPS in skin fibroblasts was: LMCH > LMAG > LMST. The different protection levels of these LMPS may be due to the physic-chemical properties of the LMPS’ functional groups, including electron transfer ability, metal ion chelating capacities, radical stabilizing capacity, and the hydrophobicity of the constituent sugars. The results suggest that LMCH might constitute a novel and potential dermal therapeutic and sun-protective agent.
Keywords: antioxidant; skin fibroblast; oxidative damage; oxidative stress; ROS; lipid peroxidation; DNA damage; agar; chitosan; starch
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Chen, S.-K.; Hsu, C.-H.; Tsai, M.-L.; Chen, R.-H.; Drummen, G.P.C. Inhibition of Oxidative Stress by Low-Molecular-Weight Polysaccharides with Various Functional Groups in Skin Fibroblasts. Int. J. Mol. Sci. 2013, 14, 19399-19415.
Chen S-K, Hsu C-H, Tsai M-L, Chen R-H, Drummen GPC. Inhibition of Oxidative Stress by Low-Molecular-Weight Polysaccharides with Various Functional Groups in Skin Fibroblasts. International Journal of Molecular Sciences. 2013; 14(10):19399-19415.
Chen, Szu-Kai; Hsu, Chu-Hsi; Tsai, Min-Lang; Chen, Rong-Huei; Drummen, Gregor P.C. 2013. "Inhibition of Oxidative Stress by Low-Molecular-Weight Polysaccharides with Various Functional Groups in Skin Fibroblasts." Int. J. Mol. Sci. 14, no. 10: 19399-19415.