Int. J. Mol. Sci. 2013, 14(9), 19086-19108; doi:10.3390/ijms140919086
Article

Adverse Impact of Diet-Induced Hypercholesterolemia on Cardiovascular Tissue Homeostasis in a Rabbit Model: Time-Dependent Changes in Cardiac Parameters

1 Department of Cardiology, Faculty of Medicine, University of Debrecen, Nagyerdei krt. 98, Debrecen 4032, Hungary 2 Department of Pharmacology, Faculty of Pharmacy, University of Debrecen, Nagyerdei krt. 98, Debrecen 4032, Hungary 3 MTA-DE Vascular Biology, Thrombosis and Hemostasis Research Group, Hungarian Academy of Sciences, University of Debrecen, Nagyerdei krt. 98, Debrecen 4032, Hungary 4 Department of Nephrology, Medical and Health Science Center, University of Debrecen, Nagyerdei krt. 98, Debrecen 4032, Hungary 5 Department of Pediatrics, University of Debrecen, Nagyerdei krt. 98, Debrecen 4032, Hungary
* Author to whom correspondence should be addressed.
Received: 1 July 2013; in revised form: 31 July 2013 / Accepted: 2 August 2013 / Published: 17 September 2013
(This article belongs to the Special Issue Oxidative Stress and Ageing)
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Abstract: The present study evaluates a hypothesis that diet-related hypercholesterolemia increases oxidative stress-related burden to cardiovascular tissue, resulting in progressively increased mortality, along with deterioration of electrophysiological and enzymatic function in rabbit myocardium. New Zealand white rabbits were divided into four groups, defined as follows: GROUP I, cholesterol-free rabbit chow for 12 weeks; GROUP II, cholesterol-free chow, 40 weeks; GROUP III, chow supplemented with 2% cholesterol, 12 weeks; GROUP IV, chow supplemented with 2% cholesterol, 40 weeks. At the 12 and 40 weeks time points, animals in each of the aforementioned cohorts were subjected to echocardiographic measurements, followed by sacrifice. Significant deterioration in major outcome variables measured in the present study were observed only in animals maintained for 40 weeks on 2% cholesterol-supplemented chow, with much lesser adverse effects noted in animals fed high cholesterol diets for only 12 weeks. It was observed that rabbits receiving high cholesterol diets for 40 weeks exhibited significantly increased mortality, worsened ejection fraction and general deterioration of cardiac functions, along with increased atherosclerotic plaque formation and infarct size. Additionally, myocardium of GROUP IV animals was observed to contain lower levels of heme oxygenase-1 (HO-1) and cytochrome c oxidase III (COX III) protein relative to the controls.
Keywords: hypercholesterolemic rabbit; cardiac parameters; echocardiography; heme-oxygenase; cytochrome oxidase; VEGF

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MDPI and ACS Style

Kertész, A.; Bombicz, M.; Priksz, D.; Balla, J.; Balla, G.; Gesztelyi, R.; Varga, B.; Haines, D.D.; Tosaki, A.; Juhasz, B. Adverse Impact of Diet-Induced Hypercholesterolemia on Cardiovascular Tissue Homeostasis in a Rabbit Model: Time-Dependent Changes in Cardiac Parameters. Int. J. Mol. Sci. 2013, 14, 19086-19108.

AMA Style

Kertész A, Bombicz M, Priksz D, Balla J, Balla G, Gesztelyi R, Varga B, Haines DD, Tosaki A, Juhasz B. Adverse Impact of Diet-Induced Hypercholesterolemia on Cardiovascular Tissue Homeostasis in a Rabbit Model: Time-Dependent Changes in Cardiac Parameters. International Journal of Molecular Sciences. 2013; 14(9):19086-19108.

Chicago/Turabian Style

Kertész, Attila; Bombicz, Mariann; Priksz, Daniel; Balla, Jozsef; Balla, Gyorgy; Gesztelyi, Rudolf; Varga, Balazs; Haines, David D.; Tosaki, Arpad; Juhasz, Bela. 2013. "Adverse Impact of Diet-Induced Hypercholesterolemia on Cardiovascular Tissue Homeostasis in a Rabbit Model: Time-Dependent Changes in Cardiac Parameters." Int. J. Mol. Sci. 14, no. 9: 19086-19108.

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