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Isolation and Characterisation of 11 Polymorphic Microsatellite Markers in Papaver rhoeas L. (Corn Poppy), a Major Annual Plant Species from Cultivated Areas
Weed Science Laboratory, Department of Pesticide Control & Phytopharmacy, Benaki Phytopathological Institute, 8 Stefanou Delta, Kifissia, 14561 Attiki, Greece
INRA, UMR1347 Agroécologie, 17 rue Sully, F-21000 Dijon, France
INRA, UMR1095 Génétique, Diversité et Écophysiologie des Céréales, 234 avenue du Brézet, F-63100 Clermont-Ferrand, France
* Author to whom correspondence should be addressed.
Received: 16 November 2012; in revised form: 7 December 2012 / Accepted: 10 December 2012 / Published: 24 December 2012
Abstract: Papaver rhoeas, an annual plant species in the Papaveraceae family, is part of the biodiversity of agricultural ecosystems and also a noxious agronomic weed. We developed microsatellite markers to study the genetic diversity of P. rhoeas, using an enriched microsatellite library coupled with 454 next-generation sequencing. A total of 13,825 sequences were obtained that yielded 1795 microsatellite loci. After discarding loci with less than six repeats of the microsatellite motif, automated primer design was successful for 598 loci. We tested 74 of these loci for amplification with a total of 97 primer pairs. Thirty loci passed our tests and were subsequently tested for polymorphism using 384 P. rhoeas plants originating from 12 populations from France. Of the 30 loci, 11 showed reliable polymorphism not affected by the presence of null alleles. The number of alleles and the expected heterozygosity ranged from 3 to 7.4 and from 0.27 to 0.73, respectively. A low but significant genetic differentiation among populations was observed (FST = 0.04; p < 0.001). The 11 validated polymorphic microsatellite markers developed in this work will be useful in studies of genetic diversity and population structure of P. rhoeas, assisting in designing management strategies for the control or the conservation of this species.
Keywords: Papaver rhoeas; Papaveraceae; microsatellite markers; next-generation sequencing; genetic diversity
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MDPI and ACS Style
Kati, V.; Le Corre, V.; Michel, S.; Jaffrelo, L.; Poncet, C.; Délye, C. Isolation and Characterisation of 11 Polymorphic Microsatellite Markers in Papaver rhoeas L. (Corn Poppy), a Major Annual Plant Species from Cultivated Areas. Int. J. Mol. Sci. 2013, 14, 470-479.
Kati V, Le Corre V, Michel S, Jaffrelo L, Poncet C, Délye C. Isolation and Characterisation of 11 Polymorphic Microsatellite Markers in Papaver rhoeas L. (Corn Poppy), a Major Annual Plant Species from Cultivated Areas. International Journal of Molecular Sciences. 2013; 14(1):470-479.
Kati, Vaya; Le Corre, Valérie; Michel, Séverine; Jaffrelo, Lydia; Poncet, Charles; Délye, Christophe. 2013. "Isolation and Characterisation of 11 Polymorphic Microsatellite Markers in Papaver rhoeas L. (Corn Poppy), a Major Annual Plant Species from Cultivated Areas." Int. J. Mol. Sci. 14, no. 1: 470-479.