Quantitative Profiling of DNA Damage and Apoptotic Pathways in UV Damaged Cells Using PTMScan Direct
Abstract
:1. Introduction
2. Results
2.1. PTMScan Direct: DNA Damage/Cell Cycle and Apoptosis/Autophagy Reagents
2.2. PTMScan Direct Profiling of UV Damage in HeLa Cells
3. Discussion
3.1. Novel PTMScan Direct Reagents
3.2. DNA Damage Response Signaling
3.3. Induction of Apoptosis
4. Experimental Section
4.1. Overview
4.2. Cell Lines
4.3. LC-MS/MS Analysis
4.4. Data Analysis
4.5. Western Blotting
5. Conclusions
Abbreviations
CST | Cell Signaling Technology |
DDR | DNA damage response |
IAP | Immunoaffinity purification |
LC-MS/MS | Liquid chromatography tandem mass spectrometry |
PI3K | Phosphatidylinositol 3-kinase |
PTM | Post-translational modification |
- Conflict of InterestThe authors declare no conflict of interest.
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Stokes, M.P.; Silva, J.C.; Jia, X.; Lee, K.A.; Polakiewicz, R.D.; Comb, M.J. Quantitative Profiling of DNA Damage and Apoptotic Pathways in UV Damaged Cells Using PTMScan Direct. Int. J. Mol. Sci. 2013, 14, 286-307. https://doi.org/10.3390/ijms14010286
Stokes MP, Silva JC, Jia X, Lee KA, Polakiewicz RD, Comb MJ. Quantitative Profiling of DNA Damage and Apoptotic Pathways in UV Damaged Cells Using PTMScan Direct. International Journal of Molecular Sciences. 2013; 14(1):286-307. https://doi.org/10.3390/ijms14010286
Chicago/Turabian StyleStokes, Matthew P., Jeffrey C. Silva, Xiaoying Jia, Kimberly A. Lee, Roberto D. Polakiewicz, and Michael J. Comb. 2013. "Quantitative Profiling of DNA Damage and Apoptotic Pathways in UV Damaged Cells Using PTMScan Direct" International Journal of Molecular Sciences 14, no. 1: 286-307. https://doi.org/10.3390/ijms14010286