Int. J. Mol. Sci. 2013, 14(1), 1952-1963; doi:10.3390/ijms14011952
Article

pH Dependence of the Fluorescence Lifetime of FAD in Solution and in Cells

Md. Serajul Islam 1 email, Masato Honma 1 email, Takakazu Nakabayashi 1,2,* email, Masataka Kinjo 3 email and Nobuhiro Ohta 1,2,* email
1 Graduate School of Environmental Science, Hokkaido University, Sapporo 060-0810, Japan 2 Research Institute for Electronic Science (RIES), Hokkaido University, Sapporo 001-0020, Japan 3 Faculty of Advanced Life Science, Hokkaido University, Sapporo 001-0021, Japan
* Authors to whom correspondence should be addressed.
Received: 21 November 2012; in revised form: 27 December 2012 / Accepted: 9 January 2013 / Published: 18 January 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: We have studied physiological parameters in a living cell using fluorescence lifetime imaging of endogenous chromophores. In this study, pH dependence of the fluorescence lifetime of flavin adenine dinucleotide (FAD), that is a significant cofactor exhibiting autofluorescence, has been investigated in buffer solution and in cells. The fluorescence lifetime of FAD remained unchanged with pH 5 to 9 in solution. However, the fluorescence lifetime in HeLa cells was found to decrease with increasing intracellular pH, suggesting that pH in a single cell can be estimated from the fluorescence lifetime imaging of FAD without adding exogenous fluorescent probes.
Keywords: FAD; autofluorescence; living cell; fluorescence lifetime imaging; intracellular pH; fluorescence decay

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MDPI and ACS Style

Islam, M.S.; Honma, M.; Nakabayashi, T.; Kinjo, M.; Ohta, N. pH Dependence of the Fluorescence Lifetime of FAD in Solution and in Cells. Int. J. Mol. Sci. 2013, 14, 1952-1963.

AMA Style

Islam MS, Honma M, Nakabayashi T, Kinjo M, Ohta N. pH Dependence of the Fluorescence Lifetime of FAD in Solution and in Cells. International Journal of Molecular Sciences. 2013; 14(1):1952-1963.

Chicago/Turabian Style

Islam, Md. S.; Honma, Masato; Nakabayashi, Takakazu; Kinjo, Masataka; Ohta, Nobuhiro. 2013. "pH Dependence of the Fluorescence Lifetime of FAD in Solution and in Cells." Int. J. Mol. Sci. 14, no. 1: 1952-1963.

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