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Int. J. Mol. Sci. 2013, 14(1), 1952-1963; doi:10.3390/ijms14011952
Article

pH Dependence of the Fluorescence Lifetime of FAD in Solution and in Cells

1
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1
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1,2,* , 3
 and
1,2,*
Received: 21 November 2012 / Revised: 27 December 2012 / Accepted: 9 January 2013 / Published: 18 January 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

We have studied physiological parameters in a living cell using fluorescence lifetime imaging of endogenous chromophores. In this study, pH dependence of the fluorescence lifetime of flavin adenine dinucleotide (FAD), that is a significant cofactor exhibiting autofluorescence, has been investigated in buffer solution and in cells. The fluorescence lifetime of FAD remained unchanged with pH 5 to 9 in solution. However, the fluorescence lifetime in HeLa cells was found to decrease with increasing intracellular pH, suggesting that pH in a single cell can be estimated from the fluorescence lifetime imaging of FAD without adding exogenous fluorescent probes.
Keywords: FAD; autofluorescence; living cell; fluorescence lifetime imaging; intracellular pH; fluorescence decay FAD; autofluorescence; living cell; fluorescence lifetime imaging; intracellular pH; fluorescence decay
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Islam, M.S.; Honma, M.; Nakabayashi, T.; Kinjo, M.; Ohta, N. pH Dependence of the Fluorescence Lifetime of FAD in Solution and in Cells. Int. J. Mol. Sci. 2013, 14, 1952-1963.

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