Abstract: We have studied physiological parameters in a living cell using fluorescence lifetime imaging of endogenous chromophores. In this study, pH dependence of the fluorescence lifetime of flavin adenine dinucleotide (FAD), that is a significant cofactor exhibiting autofluorescence, has been investigated in buffer solution and in cells. The fluorescence lifetime of FAD remained unchanged with pH 5 to 9 in solution. However, the fluorescence lifetime in HeLa cells was found to decrease with increasing intracellular pH, suggesting that pH in a single cell can be estimated from the fluorescence lifetime imaging of FAD without adding exogenous fluorescent probes.
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Islam, M.S.; Honma, M.; Nakabayashi, T.; Kinjo, M.; Ohta, N. pH Dependence of the Fluorescence Lifetime of FAD in Solution and in Cells. Int. J. Mol. Sci. 2013, 14, 1952-1963.
Islam MS, Honma M, Nakabayashi T, Kinjo M, Ohta N. pH Dependence of the Fluorescence Lifetime of FAD in Solution and in Cells. International Journal of Molecular Sciences. 2013; 14(1):1952-1963.
Islam, Md. S.; Honma, Masato; Nakabayashi, Takakazu; Kinjo, Masataka; Ohta, Nobuhiro. 2013. "pH Dependence of the Fluorescence Lifetime of FAD in Solution and in Cells." Int. J. Mol. Sci. 14, no. 1: 1952-1963.