Int. J. Mol. Sci. 2013, 14(1), 1940-1951; doi:10.3390/ijms14011940
Article

Resveratrol Down-Regulates Myosin Light Chain Kinase, Induces Apoptosis and Inhibits Diethylnitrosamine-Induced Liver Tumorigenesis in Rats

Xiao-Lin Zhang 1,†,* email, Hao Yu 1,† email, You-Yi Xiong 1 email, Shi-Tang Ma 1 email, Lei Zhao 2 email and Shi-Feng She 3,* email
1 Food and Drug College of Anhui Science and Technology University, Bengbu 233100, Anhui, China 2 Animal Science College of Anhui Science and Technology University, Bengbu 233100, Anhui, China 3 Department of Digestive Medicine, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong, China † These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 7 November 2012; in revised form: 27 December 2012 / Accepted: 31 December 2012 / Published: 17 January 2013
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Abstract: Hepatocellular carcinoma (HCC) is a serious healthcare problem worldwide because of its increasing morbidity and high mortality rates. However, our understanding of the mechanism of liver tumorigenesis remains incomplete. We report the expression of myosin light chain kinase (MLCK) in the livers of rats with diethylnitrosamine (DENA)-induced HCC and investigated the correlation between MLCK and liver tumorigenesis by observing the expression of MLCK in a rat model of HCC. HCC was induced in rats by an intraperitoneal injection of DENA, and resveratrol-treated rats were orally administered resveratrol with 50 mg/kg body weight/day. The livers of rats were excised after 20 weeks and immersed in 10% formaldehyde prior to immunohistochemical and Western blot analyses for determining the level of MLCK expression. These analyses indicated that the MLCK expression was higher in the livers of HCC rats than in normal and resveratrol-treated rats. High level of MLCK expression was responsible for proliferation and anti-apoptotic effects. However, resveratrol down-regulated the expression of MLCK, which induced cell apoptosis and inhibited liver tumorigenesis in rats with DENA-induced HCC. Our results suggest that the over expression of MLCK may be related to the development of liver tumorigenesis.
Keywords: myosin light chain kinase; liver tumorigenesis; resveratrol; apoptosis

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MDPI and ACS Style

Zhang, X.-L.; Yu, H.; Xiong, Y.-Y.; Ma, S.-T.; Zhao, L.; She, S.-F. Resveratrol Down-Regulates Myosin Light Chain Kinase, Induces Apoptosis and Inhibits Diethylnitrosamine-Induced Liver Tumorigenesis in Rats. Int. J. Mol. Sci. 2013, 14, 1940-1951.

AMA Style

Zhang X-L, Yu H, Xiong Y-Y, Ma S-T, Zhao L, She S-F. Resveratrol Down-Regulates Myosin Light Chain Kinase, Induces Apoptosis and Inhibits Diethylnitrosamine-Induced Liver Tumorigenesis in Rats. International Journal of Molecular Sciences. 2013; 14(1):1940-1951.

Chicago/Turabian Style

Zhang, Xiao-Lin; Yu, Hao; Xiong, You-Yi; Ma, Shi-Tang; Zhao, Lei; She, Shi-Feng. 2013. "Resveratrol Down-Regulates Myosin Light Chain Kinase, Induces Apoptosis and Inhibits Diethylnitrosamine-Induced Liver Tumorigenesis in Rats." Int. J. Mol. Sci. 14, no. 1: 1940-1951.

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