- freely available
Int. J. Mol. Sci. 2013, 14(1), 191-204; doi:10.3390/ijms14010191
Published: 21 December 2012
Abstract: Human skin is exposed to solar ultraviolet radiation comprising UVB (280–315 nm) and UVA (315–400 nm) on a daily basis. Within the last two decades, the molecular and cellular response to UVA/UVB and the possible effects on human health have been investigated extensively. It is generally accepted that the mutagenic and carcinogenic properties of UVB is due to the direct interaction with DNA. On the other hand, by interaction with non-DNA chromophores as endogenous photosensitizers, UVA induces formation of reactive oxygen species (ROS), which play a pivotal role as mediators of UVA-induced injuries in human skin. This review gives a short overview about relevant findings concerning the molecular mechanisms underlying UVA/UVB-induced cell death. Furthermore, we will highlight the potential role of cutaneous antioxidants and photolabile nitric oxide derivates (NODs) in skin physiology. UVA-induced decomposition of the NODs, like nitrite, leads not only to non-enzymatic formation of nitric oxide (NO), but also to toxic reactive nitrogen species (RNS), like peroxynitrite. Whereas under antioxidative conditions the generation of protective amounts of NO is favored, under oxidative conditions, less injurious reactive nitrogen species are generated, which may enhance UVA-induced cell death.
Human skin is exposed to sunlight on a daily basis, and especially, the biological effects of the ultraviolet region namely UVB (280–315 nm) and UVA (315–400 nm) have been investigated intensely in the last decades. It has been recognized that exposure to solar ultraviolet radiation has both beneficial and deleterious effects on human health and skin physiology. Apart from painful sunburns as a result of excessive sun exposure, chronic exposure to UVA and UVB is related to increased risk of skin cancer  and premature skin ageing . On the other hand, a growing body of studies and data suggest general health benefits of sunlight and UVR-exposure . In this context, in particular, the production of Vitamin D in the deeper epidermis of the skin by UVB and the resulting higher circulating concentrations of serum 25-hydroxyvitamin D may reduce the risk of many chronic and infectious diseases, inter alia, cancer, hypertension and cardiovascular diseases, autoimmune diseases and bacterial and viral infections [4–6]. Furthermore, it has been suggested that many of the beneficial effects of sunlight, particularly those related to cardiovascular health, are mediated by mechanisms that are independent of vitamin D production. For example, it has been hypothesized that photolabile nitric oxide (NO)-related species or compounds, such as nitrite and nitrosothiols, which are stored in comparably high concentration in the skin, can be mobilized by UVA and delivered to the systemic circulation, exerting coronary vasodilation and antihypertensive effects (see Figure 1) [7,8].
However, despite the controversial discussion of the beneficial and hazardous effects of sunlight, there is incontrovertible evidence that UVB, and also UVA exposure, can induce cell damage, which in turn causes necrosis or induces apoptosis  and autophagy [10,11]. Thus, we will review briefly in this article the common mechanism of UVA- and UVB-induced cell damage and highlight the possible role of NO-related species as endogenous photo-sensitizers, which, especially during UVA exposure, may represent a protective principle against UVA-induced and ROS-mediated cell and tissue damage.
2. UVR-Induced Cell Damage
An overexposure to UVB can lead to all kinds of maladies, including sunburn, skin cancer and cataracts. It is generally accepted that the mutagenic and carcinogenic properties of UVB is due to the direct interaction with DNA and consequent generation of pyrimidine dimers, namely cyclobutane pyrimidine dimers (CPDs) and pyrimidine pyrimidone photoproducts .
Studies suggest that these lesions are involved in photocarcinogenesis by the high proportion of p53 mutations (TC to TT or CC to TT transitions) detected at bipyrimidine sites in non-melanoma skin cancers  and UVB-induced skin tumors in a hairless mouse model .
Although these DNA-damages are potentially mutagenic, virtually all mammalian cells have several enzymatic systems to repair UVB-induced DNA . Once cell cycle checkpoint systems sense abnormal chromosomal DNA structures, they execute cell cycle arrest through inhibiting the activity of cell cycle regulators. This is necessary to coordinate cell division with the DNA repair process or, if the damage is too severe, execute apoptosis and premature senescence of the affected cells .
However, UVB-stress response, such as NF-kappa B activation, occurs also in enucleated cells, indicating that the response is initiated at or near the plasma membrane, rather than the nucleus . Further studies could support these findings and show that a major part of the UVB-response of cells is DNA-damage independent. As very early initiating events after UVB-exposure, the clustering and internalization of cell surface receptors for epidermal growth factor (EGF), tumor necrosis factor (TNF) and interleukin-1β (IL-1β) could be observed . As one of the responsible UVB chromophores for DNA-independent UVB-response, the arylhydrocarbon receptor (AhR) ligand 6-formylindolo[3,2-b]carbazole (FICZ) was identified in human skin cells . Recent studies have shown that UVB irradiation also induces in keratinocytes the expression of Cyclophilin D, a key component for opening mitochondrial permeability transition pores, leading to both apoptopic and necrotic cell death . As reported recently, UVB irradiation modificates major lipid raft components in skin cells by increasing cholesterol levels in membrane rafts, which leads to Fas-receptor aggregation and to subsequent events as the formation of death-inducing signaling complex and apoptosis . On the other hand, it has been demonstrated that UVB irradiation leads also to an increase of the protein prohibitin in membrane rafts, which may have a protective function against UVB-induced apoptosis . Apart from the UVA-induced effects on DNA and cell membranes, UVB affects miRNA expression profiles in mouse epidermis, which could be associated with photocarcinogenesis and apoptosis . Moreover, UVB as well as UVA can induce damage of proteins mainly by interaction with amino acid residues, such as tryptophan, tyrosine, histidine and cystine, via both excited state species and radicals .
To sum up, UVB response in the skin is a multifaceted biological process and originates from multiple intracellular sites. Besides the above mentioned mechanism, UVB can induce also the formation of reactive oxygen species  and affects or damages proteins by direct oxidation or by covalent binding of lipid peroxidation breakdown products [26,27].
The amount of UVA radiation on earth’s surface is approximately 20-fold higher than that of UVB. It penetrates deeper (up to 1 mm) into the skin in comparison to UVB (20 μM) and can reach the dermis . UVA is responsible for the tanning effects of human skin and had been considered mostly harmless for many years. However, studies have shown that UVA contributes to photoaging, photocarcinogenesis and photodermatosis [29,30] by photooxidative mechanisms and the formation of reactive oxygen species (ROS) . Furthermore, apart from UVB radiation, UVA radiation can also induce the generation of bipyrimidine photoproducts, respectively, cyclobutane pyrimidine dimers (CPDs). It seems now most likely that UVA-induced CPDs arise from a direct photoreaction rather than photosensitizer-mediated processes . The formation of ROS, for example, oxygen radicals and singlet oxygen seems to be dependent on non-DNA chromophores, such as porphyrins, bilirubin, melanin and flavins, acting as endogenous photosensitizers. Excessive ROS formation in a cell leads to the damage of many biomolecules, including DNA and membrane lipids and, thus, cytotoxicity, mutations and alterations in cell signaling pathways . Given solar spectral distribution, solar UVA radiation is much more effective in triggering lipid peroxidation than UVB . UVA-induced oxidative stress provokes an immediate increase in the available pool of intracellular redox-active and chelatable iron ions, leading to the catalytic formation of more oxygen-derived free radicals that may overwhelm the cellular antioxidant defense, with subsequent cell damage .
Regarding UVA-induced cell membrane damage, it had been shown that the radical nitric oxide can inhibit lipid peroxidation by scavenging lipid peroxyl radicals and/or inhibiting initiators of lipid peroxidation, respectively, and, consequently, also inhibits UVA-induced cell death via apoptosis or necrosis [36–40]. However, NO may react with superoxide to peroxynitrite, which initiates lipid peroxidation itself and oxidizes lipid soluble antioxidants .
Therefore, the next sections will focus on the involvement of NO and photolabile NO-related species in the physiological response of the skin to UVA-exposure.
3. Physiology of Nitric Oxide
Nitric oxide (NO) plays a pivotal role in human’s physiology and pathophysiology [42–44]. It is the smallest known bioactive product of mammalian cells, is highly diffusible and reactive and can be produced by most cell types. Although ubiquitous, this simple molecule can act very specifically, controlling vital functions, such as neurotransmission and vascular tone via activation of soluble guanylyl cyclase [45,46], gene transcription  and mRNA translation (via iron-responsive elements) . It can generate posttranslational modifications of proteins (via ADP-ribosylation)  and is capable of destroying tumor cells and parasites by inhibiting iron-containing enzymes  or interacting directly with the DNA of these cells . Nitric oxide plays an important role in skin physiology as well and is involved in wound healing , keratinocyte proliferation and differentiation  and cutaneous inflammation and immune reactions . In addition, several lines of evidence indicate that NO regulates the expression of protective stress response genes, such as vascular endothelial growth factor (VEGF) and heme oxygenase (HO)-1 or Bcl-2 [38,55,56]. Furthermore, NO can inhibit UVA-induced lipid peroxidation [41,57], and the coordinated effects of NO on gene expression and preservation of membrane integrity effectively protect against UVA- and reactive oxygen species (ROS)-induced apoptotic as well as necrotic cell death .
In the human body, NO is formed endogenously by three NO synthase enzymes . The keratinocytes express the neuronal isoform of NO synthase (nNOS), whereas the fibroblasts and other cell types in the skin express the endothelial isoform (eNOS). This constitutive low level NO production in the skin seems to play a role in the maintenance of barrier function and in determining blood flow . The inducible isoform of NO synthase (iNOS) is not expressed usually in the skin, but under certain conditions, virtually all skin cells are capable of expressing iNOS. The following high level production of NO is often correlated with psoriasis and other inflammatory skin conditions . The irradiation of the skin by UVB and/or UVA induces in the skin the release of inflammation transmitters, like IL-1, IL-10, TGF-β1, TNF-α, which induce iNOS to produce higher NO-concentration, leading to erythema, edema and stimulation of melanogenesis (for review see ). However, we could show that iNOS can be induced by UVA in the absence of proinflamatory cytokines . Hereinafter, we will explain the many functions of NO and highlight the potential role of naturally occurring NODs in the biological response to UVA challenge by non-enzymatic formation of NO.
4. Nitrogen Oxide Derivates as Source of Nitric Oxide
A part of the NO molecules generated in skin reacts rapidly with oxygen or proteins. The products of these reactions are mainly S- or N-nitroso compounds (RSNOs or RNNOs) and the oxidation products nitrite or nitrate. These nitrogen oxide derivates (NODs) stay close to the place of their origin and accumulate this way in cells and tissue. Thus, comparably high NOD concentrations can be found in human skin (nitrite up to 15 μM, nitroso compounds up to 7 μM and nitrate up to 100 μM) .
Additionally to the enzymatic NO formation as described above, NO can also be formed non-enzymatically, e.g., by pH-catalyzed and UVA-induced decomposition of NODs, namely nitrite, nitrate and S-nitroso compounds (RSNOs), such as S-nitrosoalbumin, S-nitrosoglutathione or S-nitrosocysteine.
Already in the 1960s, the pioneers of nitric oxide research, Furchgott and Ehrreich, accidentally made the observation that daylight irradiation of blood vessels induces dilation . This effect called photorelaxtion was markedly enhanced by nitrite solutions, indicating that under certain circumstances, nitrite may exhibit relaxation activities comparable to NO.
Indeed, studies in environmental chemistry revealed that the N-O-bond of the nitrite ion in aqueous solution will be disrupted by the energy of light within the UVA spectrum at 340–360 nm (see Figure 2).
This UVA-induced photodecomposition of nitrite results in a constant low-level formation of NO and also of various reactive oxygen and nitrogen species . In comparison, the irradiation of RSNOs results in a higher, but short-lasting, NO-formation, accompanied by the rapid loss of these nitroso species . Nitrate is generally considered as a very inert oxidative product of NO. But it could be shown that limited amounts of NO are produced during UV-irradiation of nitrate, which is enhanced in the presence of thiols .
However, we consider nitrite as the predominant source of NO during UVA exposure of the human skin. In the next sections, we will describe the underlying mechanism of UVA-induced nitrite decomposition, its rule in cell death and protection and how antioxidants affect the yield of bioactive NO.
5. UVA Induces Non-Enzymatic NO-Formation in Human Skin
Nitric oxide derivates are present in blood as products of enzymatic NO synthesis and as a consequence of the intake of nitrite/nitrate-containing food. In normal human skin, we found nitrite and S-nitrosothiols (RSNO) at concentrations 25- or 360-fold higher than those found in the plasma of healthy volunteers . This finding could be confirmed by Mowbray et al.  using other methods for the determination of cutaneous NODs.
The challenge for normal human skin samples with UVA at doses equivalent to only 3–5 min of sun exposure in the Central European summer (approx. 5 mW/cm2) leads to significant non-enzymatic NO-formation due to the decomposition of nitrite and RSNOs . This result could be found also in vivo by Mowbray et al.  and by ourselves in further studies, where we measured in vivo the enzyme-independent generation of gaseous NO directly above the skin surface . Surprisingly, nitrate does not contribute to UVA-provoked NO release from human skin.
Previously, we had shown that endogenously produced iNOS-derived NO and exogenously applied NO shows protective activity against UVA-induced cell death in endothelial cells [38,39]. Further studies showed that also the presence of nitrite exerts NO-dependent protection against UVA-induced cell death in endothelial cells .
The skin is frequently exposed to UVA irradiation and UVA especially reaches the dermis and causes photo-oxidative stress in the resident fibroblasts. The purpose of further studies was to examine whether nitrite or other NODs may exhibit a similar protective NO-dependent activity against UVA-induced cell death in fibroblasts as in endothelial cells. In contrast to endothelial cells, we found that the presence of exogenous nitrite enhances the UVA-induced lipid peroxidation and the susceptibility to toxic effects of UVA in dermal fibroblast in a concentration-dependent manner .
The mechanism of UVA-induced nitrite decomposition (Reactions 1–5) reveals the initial generation of NO and a cascade of further reactions leading to nitrosative stress by generation of the highly toxic nitrogen dioxide NO2·, which is capable of initiating the lipid peroxidation chain reaction.
Furthermore, nitrogen dioxide consumes NO (Reaction 4), which is an efficient inhibitor of lipid peroxidation. Thus, Reaction (4) further supports the injurious effects of UVA-induced nitrite decomposition. Consequently, ascorbic acid, a potent nitrogen dioxide antagonist , protects against UVA/nitrite-induced lipid peroxidation and toxicity by scavenging nitrogen dioxide and by the simultaneous enhancement of UVA-induced NO formation from nitrite. Thus, the oxidative milieu is crucial for the outcome of UVA-induced nitrite decomposition and its effects on cell viability . An overview is given in Figure 3.
However, other antioxidants, like glutathione and trolox, a water-soluble vitamin E derivate, are also capable of enhancing the NO-formation from UVA-induced nitrite decomposition by many fold (see Figure 4).
But, in contrast to ascorbic acid, the presence of glutathione or trolox leads to an increase of UVA/nitrite-induced necrotic cell death. The reaction of glutathione with nitrogen dioxide preferably produces sulfur-centered glutathionyl radicals, which have been shown to promote the oxidation of phospholipids . The initial product of the reaction of nitrogen dioxide with α-tocopherol, the α-tocopheroxyl radical, is an effective lipid peroxidation-inducing agent in living cells and causes cell death [72,73].
On the other hand, the addition of NO can reverse these toxic effects. In conclusion, oxidative and nitrosative stress can result from an imbalance of prooxidants and antioxidants with excessive, destructive free-radical chemistry. Here, it seems that endothelial cells have a higher antioxidative capacity against reactive nitrogen species than fibroblasts. With respect to the possible high concentration of nitrite in sweat and the skin surface, the use of vitamin E containing creams before UVA exposure may be a potential hazard to the skin.
6. The Protective Role of Intracellular Nitrite during UVA-Challenge
Although the presence of supraphysiological concentrations of nitrite during UVA exposure shows enhanced toxicity in the cell culture of human dermal fibroblasts, nothing is known about the impact of intracellular naturally occurring NODs.
With respect to the important role of NO in human cutaneous physiology and the possible protective activity of NO, we examined the influence of intracellularly-present photolabile NODs on the UVA-induced toxicity of human skin fibroblasts .
We could show an intracellular NO-formation during UVA-irradiation, which is dependent on the intracellular NOD-concentration. We found that intracellular photolabile NODs, generally nitrite, modulate UVA-induced cell damage. A depletion of intracellular NODs leads to a decrease of intracellular NO-formation, accompanied by an increase of lipid peroxidation and UVA-induced cell toxicity via necrotic cell death. Supplementation with nitrite in physiological concentrations can reverse completely the depletion and the increased susceptibility of NOD-depleted fibroblasts cultures to the injurious effects of UVA. The use of cPTIO as NO-scavenger proves the protective role of NO produced non-enzymatically by nitrite decomposition.
These findings indicate that non-enzymatic NO formation due to decomposition of intracellularly occurring photolabile NODs represents an elementary and fast reacting physiological principle of protection against UVA-induced lipid peroxidation and cell death (see Figure 5). Additionally, studies have shown that NO may inhibit autophagy of cells . Since UVB irradiation can induce autophagy in epidermal cells, theoretically, the UVA-induced generation of NO might protect also against a possible UVB-induced autophagy in skin cells  during exposure to sun light.
In conclusion, targeting dermal NODs by pharmacological or dietary interventions may represent an additional approach in preventing UVA-, and maybe also UVB-, induced skin damage. However, further studies are necessary for the investigation of the role of nitrite and other NODs in skin physiology, their potential use as agents for sun screens and if a nitrite/nitrate enriched diet can influence the dermal NOD-stores and the UVA/UVB-response.
This work was supported by the “START” program grant provided by the Faculty of Medicine of the RWTH Aachen University (AZ3 5/12 to Christian Opländer).
- Rigel, D.S. Cutaneous ultraviolet exposure and its relationship to the development of skin cancer. J. Am. Acad. Dermatol 2008, 58, S129–S132.
- Helfrich, Y.R.; Sachs, D.L.; Voorhees, J.J. Overview of skin aging and photoaging. Dermatol. Nurs. 2008, 20, 177–183. quiz 184.
- Moan, J.; Porojnicu, A.C.; Dahlback, A.; Setlow, R.B. Addressing the health benefits and risks, involving vitamin D or skin cancer, of increased sun exposure. Proc. Natl. Acad. Sci. USA 2008, 105, 668–673.
- Van der Rhee, H.J.; de Vries, E.; Coebergh, J.W.W. Does sunlight prevent cancer? A systematic review. Eur. J. Cancer 2006, 42, 2222–2232.
- Grant, W.B.; Boucher, B.J. Requirements for vitamin D across the life span. Biol. Res. Nurs 2011, 13, 120–133.
- Brennan, P.J.; Greenberg, G.; Miall, W.E.; Thompson, S.G. Seasonal variation in arterial blood pressure. Br. Med. J. (Clin. Res. Ed.) 1982, 285, 919–923.
- Feelisch, M.; Kolb-Bachofen, V.; Liu, D.; Lundberg, J.O.; Revelo, L.P.; Suschek, C.V.; Weller, R.B. Is sunlight good for our heart? Eur. Heart J 2010, 31, 1041–1045.
- Oplander, C.; Volkmar, C.M.; Paunel-Gorgulu, A.; van Faassen, E.E.; Heiss, C.; Kelm, M.; Halmer, D.; Murtz, M.; Pallua, N.; Suschek, C.V. Whole body UVA irradiation lowers systemic blood pressure by release of nitric oxide from intracutaneous photolabile nitric oxide derivates. Circ. Res 2009, 105, 1031–1040.
- Pustisek, N.; Situm, M. UV-radiation, apoptosis and skin. Coll. Antropol 2012, 35, 339–341.
- Yang, Y.; Wang, H.; Wang, S.; Xu, M.; Liu, M.; Liao, M.; Frank, J.A.; Adhikari, S.; Bower, K.A.; Shi, X.; et al. GSK3beta signaling is involved in ultraviolet B-induced activation of autophagy in epidermal cells. Int. J. Oncol 2012, 41, 1782–1788.
- Lamore, S.D.; Wondrak, G.T. Autophagic-lysosomal dysregulation downstream of cathepsin B inactivation in human skin fibroblasts exposed to UVA. Photochem. Photobiol. Sci 2012, 11, 163–172.
- Matsunaga, T.; Hieda, K.; Nikaido, O. Wavelength dependent formation of thymine dimers and (6-4) photoproducts in DNA by monochromatic ultraviolet light ranging from 150 to 365 nm. Photochem. Photobiol 1991, 54, 403–410.
- Ziegler, A.; Leffell, D.J.; Kunala, S.; Sharma, H.W.; Gailani, M.; Simon, J.A.; Halperin, A.J.; Baden, H.P.; Shapiro, P.E.; Bale, A.E.; et al. Mutation hotspots due to sunlight in the p53 gene of nonmelanoma skin cancers. Proc. Natl. Acad. Sci. USA 1993, 90, 4216–4220.
- Dumaz, N.; van Kranen, H.J.; de Vries, A.; Berg, R.J.; Wester, P.W.; van Kreijl, C.F.; Sarasin, A.; Daya-Grosjean, L.; de Gruijl, F.R. The role of UV-B light in skin carcinogenesis through the analysis of p53 mutations in squamous cell carcinomas of hairless mice. Carcinogenesis 1997, 18, 897–904.
- Marrot, L.; Meunier, J.R. Skin DNA photodamage and its biological consequences. J. Am. Acad. Dermatol 2008, 58, S139–S148.
- Nakanishi, M.; Niida, H.; Murakami, H.; Shimada, M. DNA damage responses in skin biology—implications in tumor prevention and aging acceleration. J. Dermatol. Sci 2009, 56, 76–81.
- Devary, Y.; Rosette, C.; DiDonato, J.A.; Karin, M. NF-kappa B activation by ultraviolet light not dependent on a nuclear signal. Science 1993, 261, 1442–1445.
- Rosette, C.; Karin, M. Ultraviolet light and osmotic stress: Activation of the JNK cascade through multiple growth factor and cytokine receptors. Science 1996, 274, 1194–1197.
- Fritsche, E.; Schafer, C.; Calles, C.; Bernsmann, T.; Bernshausen, T.; Wurm, M.; Hubenthal, U.; Cline, J.E.; Hajimiragha, H.; Schroeder, P.; et al. Lightening up the UV response by identification of the arylhydrocarbon receptor as a cytoplasmatic target for ultraviolet B radiation. Proc. Natl. Acad. Sci. USA 2007, 104, 8851–8856.
- Ji, C.; Yang, B.; Yang, Z.; Tu, Y.; Yang, Y.L.; He, L.; Bi, Z.G. Ultra-violet B (UVB)-induced skin cell death occurs through a cyclophilin D intrinsic signaling pathway. Biochem. Biophys. Res. Commun 2012, 425, 825–829.
- George, K.S.; Elyassaki, W.; Wu, Q.; Wu, S. The role of cholesterol in UV light B-induced apoptosis. Photochem. Photobiol 2012, 88, 1191–1197.
- Wu, Q.; Wu, S. Lipid rafts association and anti-apoptotic function of prohibitin in ultraviolet B light-irradiated HaCaT keratinocytes. Exp. Dermatol 2012, 21, 640–642.
- Zhou, B.R.; Xu, Y.; Luo, D. Effect of UVB irradiation on microRNA expression in mouse epidermis. Oncol. Lett 2012, 3, 560–564.
- Pattison, D.I.; Rahmanto, A.S.; Davies, M.J. Photo-oxidation of proteins. Photochem. Photobiol. Sci 2012, 11, 38–53.
- Paz, M.L.; Gonzalez Maglio, D.H.; Weill, F.S.; Bustamante, J.; Leoni, J. Mitochondrial dysfunction and cellular stress progression after ultraviolet B irradiation in human keratinocytes. Photodermatol. Photoimmunol. Photomed 2008, 24, 115–122.
- Punnonen, K.; Puntala, A.; Jansen, C.T.; Ahotupa, M. UVB irradiation induces lipid peroxidation and reduces antioxidant enzyme activities in human keratinocytes in vitro. Acta Derm. Venereol 1991, 71, 239–242.
- Stadtman, E.R.; Berlett, B.S. Reactive oxygen-mediated protein oxidation in aging and disease. Drug Metab. Rev 1998, 30, 225–243.
- Polefka, T.G.; Meyer, T.A.; Agin, P.P.; Bianchini, R.J. Effects of solar radiation on the skin. J. Cosmet. Dermatol 2012, 11, 134–143.
- Uitto, J.; Bernstein, E.F. Molecular mechanisms of cutaneous aging: Connective tissue alterations in the dermis. J. Investig. Dermatol. Symp. Proc 1998, 3, 41–44.
- Krutmann, J. Ultraviolet A radiation-induced biological effects in human skin: Relevance for photoaging and photodermatosis. J. Dermatol. Sci 2000, 23, S22–S26.
- Wondrak, G.T.; Jacobson, M.K.; Jacobson, E.L. Endogenous UVA-photosensitizers: Mediators of skin photodamage and novel targets for skin photoprotection. Photochem. Photobiol. Sci 2006, 5, 215–237.
- Cadet, J.; Mouret, S.P.; Ravanat, J.-L.; Douki, T. Photoinduced Damage to Cellular DNA: Direct and Photosensitized Reactions. Photochem. Photobiol 2012, 88, 1048–1065.
- McMillan, T.J.; Leatherman, E.; Ridley, A.; Shorrocks, J.; Tobi, S.E.; Whiteside, J.R. Cellular effects of long wavelength UV light (UVA) in mammalian cells. J. Pharm. Pharmacol 2008, 60, 969–976.
- Morliere, P.; Moysan, A.; Tirache, I. Action spectrum for UV-induced lipid peroxidation in cultured human skin fibroblasts. Free Radic. Biol. Med 1995, 19, 365–371.
- Aroun, A.; Zhong, J.L.; Tyrrell, R.M.; Pourzand, C. Iron, oxidative stress and the example of solar ultraviolet A radiation. Photochem. Photobiol. Sci 2012, 11, 118–134.
- Suschek, C.V.; Paunel, A.; Kolb-Bachofen, V. Nonenzymatic nitric oxide formation during UVA irradiation of human skin: Experimental setups and ways to measure. Methods Enzymol 2005, 396, 568–578.
- Suschek, C.V.; Schroeder, P.; Aust, O.; Sies, H.; Mahotka, C.; Horstjann, M.; Ganser, H.; Murtz, M.; Hering, P.; Schnorr, O.; et al. The presence of nitrite during UVA irradiation protects from apoptosis. FASEB J 2003, 17, 2342–2344.
- Suschek, C.V.; Briviba, K.; Bruch-Gerharz, D.; Sies, H.; Kroncke, K.D.; Kolb-Bachofen, V. Even after UVA-exposure will nitric oxide protect cells from reactive oxygen intermediate-mediated apoptosis and necrosis. Cell Death Differ 2001, 8, 515–527.
- Suschek, C.V.; Krischel, V.; Bruch-Gerharz, D.; Berendji, D.; Krutmann, J.; Kroncke, K.D.; Kolb-Bachofen, V. Nitric oxide fully protects against UVA-induced apoptosis in tight correlation with Bcl-2 up-regulation. J. Biol. Chem 1999, 274, 6130–6137.
- Suschek, C.V.; Oplander, C.; van Faassen, E.E. Non-enzymatic NO production in human skin: Effect of UVA on cutaneous NO stores. Nitric Oxide 2010, 22, 120–135.
- Hogg, N.; Kalyanaraman, B. Nitric oxide and lipid peroxidation. Biochim. Biophys. Acta 1999, 1411, 378–384.
- Kroncke, K.D.; Fehsel, K.; Suschek, C.; Kolb-Bachofen, V. Inducible nitric oxide synthase-derived nitric oxide in gene regulation, cell death and cell survival. Int. Immunopharmacol 2001, 1, 1407–1420.
- Kroncke, K.D.; Klotz, L.O.; Suschek, C.V.; Sies, H. Comparing nitrosative versus oxidative stress toward zinc finger-dependent transcription. Unique role for NO. Nitric Oxide 2002, 277, 13294–13301.
- Kroncke, K.D.; Suschek, C.V.; Kolb-Bachofen, V. Implications of inducible nitric oxide synthase expression and enzyme activity. Antioxid. Redox Signal 2000, 2, 585–605.
- Snyder, S.H. Nitric oxide: First in a new class of neurotransmitters. Science 1992, 257, 494–496.
- Forstermann, U.; Closs, E.I.; Pollock, J.S.; Nakane, M.; Schwarz, P.; Gath, I.; Kleinert, H. Nitric oxide synthase isozymes. Characterization, purification, molecular cloning, and functions. Hypertension 1994, 23, 1121–1131.
- Peunova, N.; Enikolopov, G. Amplification of calcium-induced gene transcription by nitric oxide in neuronal cells. Nature 1993, 364, 450–453.
- Weiss, G.; Goossen, B.; Doppler, W.; Fuchs, D.; Pantopoulos, K.; Werner-Felmayer, G.; Wachter, H.; Hentze, M.W. Translational regulation via iron-responsive elements by the nitric oxide/NO-synthase pathway. EMBO J 1993, 12, 3651–3657.
- Brune, B.; Dimmeler, S.; Molina y Vedia, L.; Lapetina, E.G. Nitric oxide: A signal for ADP-ribosylation of proteins. Life Sci 1994, 54, 61–70.
- Nathan, C.F.; Hibbs, J.B., Jr. Role of nitric oxide synthesis in macrophage antimicrobial activity. Curr. Opin. Immunol. 1991, 3, 65–70.
- Wink, D.A.; Kasprzak, K.S.; Maragos, C.M.; Elespuru, R.K.; Misra, M.; Dunams, T.M.; Cebula, T.A.; Koch, W.H.; Andrews, A.W.; Allen, J.S.; et al. DNA deaminating ability and genotoxicity of nitric oxide and its progenitors. Science 1991, 254, 1001–1003.
- Frank, S.; Kampfer, H.; Wetzler, C.; Pfeilschifter, J. Nitric oxide drives skin repair: Novel functions of an established mediator. Kidney Int. 2002, 61, 882–888.
- Krischel, V.; Bruch-Gerharz, D.; Suschek, C.; Kroncke, K.D.; Ruzicka, T.; Kolb-Bachofen, V. Biphasic effect of exogenous nitric oxide on proliferation and differentiation in skin derived keratinocytes but not fibroblasts. J. Invest. Dermatol 1998, 111, 286–291.
- Bruch-Gerharz, D.; Ruzicka, T.; Kolb-Bachofen, V. Nitric oxide in human skin: Current status and future prospects. J. Invest. Dermatol 1998, 110, 1–7.
- Ehrt, S.; Schnappinger, D.; Bekiranov, S.; Drenkow, J.; Shi, S.; Gingeras, T.R.; Gaasterland, T.; Schoolnik, G.; Nathan, C. Reprogramming of the macrophage transcriptome in response to interferon-gamma and Mycobacterium tuberculosis: Signaling roles of nitric oxide synthase-2 and phagocyte oxidase. J. Exp. Med 2001, 194, 1123–1140.
- Hemish, J.; Nakaya, N.; Mittal, V.; Enikolopov, G. Nitric oxide activates diverse signaling pathways to regulate gene expression. J. Biol. Chem 2003, 278, 42321–42329.
- Niziolek, M.; Korytowski, W.; Girotti, A.W. Nitric oxide-induced resistance to lethal photooxidative damage in a breast tumor cell line. Free Radic. Biol. Med 2006, 40, 1323–1331.
- Moncada, S.; Higgs, A. The L-arginine-nitric oxide pathway. N. Engl. J. Med 1993, 329, 2002–2012.
- Warren, J.B. Nitric oxide and human skin blood flow responses to acetylcholine and ultraviolet light. FASEB J 1994, 8, 247–251.
- Weller, R.; Dykhuizen, R.; Leifert, C.; Ormerod, A. Nitric oxide release accounts for the reduced incidence of cutaneous infections in psoriasis. J. Am. Acad. Dermatol 1997, 36, 281–282.
- Cals-Grierson, M.M.; Ormerod, A.D. Nitric oxide function in the skin. Nitric Oxide 2004, 10, 179–193.
- Suschek, C.V.; Bruch-Gerharz, D.; Kleinert, H.; Forstermann, U.; Kolb-Bachofen, V. Ultraviolet A1 radiation induces nitric oxide synthase-2 expression in human skin endothelial cells in the absence of proinflammatory cytokines. J. Invest. Dermatol 2001, 117, 1200–1205.
- Paunel, A.N.; Dejam, A.; Thelen, S.; Kirsch, M.; Horstjann, M.; Gharini, P.; Murtz, M.; Kelm, M.; de Groot, H.; Kolb-Bachofen, V.; et al. Enzyme-independent nitric oxide formation during UVA challenge of human skin: Characterization, molecular sources, and mechanisms. Free Radic. Biol. Med 2005, 38, 606–615.
- Ehrreich, S.J.; Furchgott, R.F. Relaxation of mammalian smooth muscles by visible and ultraviolet radiation. Nature 1968, 218, 682–684.
- Fischer, M.; Warneck, P. Photodecomposition of nitrite and undissociated nitrous acid in aqueous solution. J. Phys. Chem 1996, 100, 18749–18756.
- Zhelyaskov, V.R.; Gee, K.R.; Godwin, D.W. Control of NO concentration in solutions of nitrosothiol compounds by light. Photochem. Photobiol 1998, 67, 282–288.
- Dejam, A.; Kleinbongard, P.; Rassaf, T.; Hamada, S.; Gharini, P.; Rodriguez, J.; Feelisch, M.; Kelm, M. Thiols enhance NO formation from nitrate photolysis. Free Radic. Biol. Med 2003, 35, 1551–1559.
- Mowbray, M.; McLintock, S.; Weerakoon, R.; Lomatschinsky, N.; Jones, S.; Rossi, A.G.; Weller, R.B. Enzyme-independent NO stores in human skin: quantification and influence of UV radiation. J. Invest. Dermatol 2009, 129, 834–842.
- Oplander, C.; Cortese, M.M.; Korth, H.-G.; Kirsch, M.; Mahotka, C.; Wetzel, W.; Pallua, N.; Suschek, C.V. The impact of nitrite and antioxidants on ultraviolet-A-induced cell death of human skin fibroblasts. Free Radic. Biol. Med 2007, 43, 818–829.
- Kirsch, M.; Korth, H.G.; Sustmann, R.; de Groot, H. The pathobiochemistry of nitrogen dioxide. Biol. Chem 2002, 383, 389–399.
- Borisenko, G.G.; Martin, I.; Zhao, Q.; Amoscato, A.A.; Tyurina, Y.Y.; Kagan, V.E. Glutathione propagates oxidative stress triggered by myeloperoxidase in HL-60 cells. Evidence for glutathionyl radical-induced peroxidation of phospholipids and cytotoxicity. J. Biol. Chem 2004, 279, 23453–23462.
- Volkmar, C.M.; Vukadinovic-Walter, B.; Oplander, C.; Bozkurt, A.; Korth, H.G.; Kirsch, M.; Mahotka, C.; Pallua, N.; Suschek, C.V. UVA-induced phenoxyl radical formation: A new cytotoxic principle in photodynamic therapy. Free Radic. Biol. Med 2010, 49, 1129–1137.
- Alessi, M.; Paul, T.; Scaiano, J.C.; Ingold, K.U. The contrasting kinetics of peroxidation of vitamin E-containing phospholipid unilamellar vesicles and human low-density lipoprotein. J. Am. Chem. Soc 2002, 124, 6957–6965.
- Oplander, C.; Wetzel, W.; Cortese, M.M.; Pallua, N.; Suschek, C.V. Evidence for a physiological role of intracellularly occurring photolabile nitrogen oxides in human skin fibroblasts. Free Radic. Biol. Med 2008, 44, 1752–1761.
- Sarkar, S.; Korolchuk, V.I.; Renna, M.; Imarisio, S.; Fleming, A.; Williams, A.; Garcia-Arencibia, M.; Rose, C.; Luo, S.; Underwood, B.R.; et al. Complex inhibitory effects of nitric oxide on autophagy. Mol. Cell 2011, 43, 19–32.
© 2013 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).