Int. J. Mol. Sci. 2013, 14(1), 1763-1777; doi:10.3390/ijms14011763
Article

Study of the UTMD-Based Delivery System to Induce Cervical Cancer Cell Apoptosis and Inhibit Proliferation with shRNA targeting Survivin

1,* email, 2email, 1email and 1email
Received: 3 November 2012; in revised form: 4 January 2013 / Accepted: 6 January 2013 / Published: 16 January 2013
(This article belongs to the Section Molecular Diagnostics)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Apoptosis induction by short hairpin RNA (shRNA) expression vectors could be an efficient and promising strategy for cancer gene therapy. Ultrasound-targeted microbubble destruction (UTMD) is an appealing technique. In this study, we investigated the apoptosis induction and suppression of cell proliferation in vivo transfected by the UTMD-based shRNA delivery system. Nude mice with transplanted tumors of cervical cancer were randomly arranged into three groups: control group, plasmid injection and ultrasound (P + US), P + UTMD group. Expressions of Survivin and proliferating cell nuclear antigen (PCNA), Bcl-2, Bax, Caspase-3, Ki-67, nucleostemin (NS) were investigated by immunohistochemistry. Furthermore, microvessel density (MVD) was detected by CD34 protein expressions and apoptotic index (AI) was measured by TUNEL. As compared with those in the control and P + US groups, protein expressions of PCNA, Ki-67, Bcl-2, Survivin and NS in P + UTMD groups were down-regulated markedly, while those of Bax, Caspase-3 were up-regulated significantly (p < 0.05). MVD decreased significantly, whereas AI increased remarkably (p < 0.05). We suggested that UTMD-based shRNA delivery system could induce apoptosis and inhibit proliferation significantly, without causing any apparently adverse effect, representing a new, promising technology that would be used in the future gene therapy and research.
Keywords: ultrasound; microbubble; apoptosis; gene therapy; RNA interference; non-viral vector
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MDPI and ACS Style

Chen, Z.-Y.; Liang, K.; Lin, Y.; Yang, F. Study of the UTMD-Based Delivery System to Induce Cervical Cancer Cell Apoptosis and Inhibit Proliferation with shRNA targeting Survivin. Int. J. Mol. Sci. 2013, 14, 1763-1777.

AMA Style

Chen Z-Y, Liang K, Lin Y, Yang F. Study of the UTMD-Based Delivery System to Induce Cervical Cancer Cell Apoptosis and Inhibit Proliferation with shRNA targeting Survivin. International Journal of Molecular Sciences. 2013; 14(1):1763-1777.

Chicago/Turabian Style

Chen, Zhi-Yi; Liang, Kun; Lin, Yan; Yang, Feng. 2013. "Study of the UTMD-Based Delivery System to Induce Cervical Cancer Cell Apoptosis and Inhibit Proliferation with shRNA targeting Survivin." Int. J. Mol. Sci. 14, no. 1: 1763-1777.

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