Buckwheat (Fagopyrum esculentum M.) Sprout Treated with Methyl Jasmonate (MeJA) Improved Anti-Adipogenic Activity Associated with the Oxidative Stress System in 3T3-L1 Adipocytes
Abstract
:1. Introduction
2. Results and Discussion
2.1. Total Phenolic Compounds and Antioxidant Capacity of Buckwheat Sprouts Extracts
2.2. Cell Viability of Buckwheat Sprouts Extracts in 3T3-L1 Preadipocytes
2.3. TBWE Suppresses the Expression of Adipocyte Markers
2.4. The Effect of TBWE on Lipid Accumulation and ROS Production in 3T3-L1 Cells
2.5. Discussion
3. Experimental Section
3.1. Materials
3.2. Determination of Total Content of Phenolic Compounds
3.3. The Ferric Reducing Power (FRAP) Assay
3.4. The Oxygen Radical Absorbance Capacity (ORAC) Assay
3.5. Cultivation and Extraction of Buckwheat Sprout Treated with 0.1 mM MeJA
3.6. Cell Culture
3.7. Cell Viability Assay
3.8. Determination of Lipid Accumulation
3.9. ROS Production in 3T3-L1 Preadipocyte
3.10. RNA Extraction and Semi-Quantitative RT-PCR
3.11. Statistical Analysis
4. Conclusions
Acknowledgments
- Conflict of InterestThe authors declare no conflict of interest.
References
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Total phenolic compound (mg/g) | Rutin (mg/g) | FRAP Assay (mM Fe2+/g of dried sample) | ORAC Values (μM trolox) | |||
---|---|---|---|---|---|---|
Hydrophilic | Lipophilic | Total | ||||
NBWE | 14.4 ± 0.4 | 2.09 ± 0.27 | 0.09 ± 0.01 | 60.6 ± 3.8 | 104.0 ± 5.1 | 164.6 ± 6.3 |
TBWE | 22.9 ± 0.6 ** | 2.99 ± 0.11 ** | 0.21 ± 0.02 ** | 103.3 ± 19.2 * | 102.5 ± 7.0 | 205.8 ± 25.8 |
Primers | Sequences | |
---|---|---|
Forward | Reverse | |
PPARγ | CCAGAGTCTGCTGATCTGCG | GCCACCTCTTTGCTCTGATC |
CEBPα | GGTGCGCAAGAGCCGAGATAAAG | AGTTCACGGCTCAGCTGTTCCAC |
aP2 | GACCTGGAAACTCGTCTCCA | CATGACACATTCCACCACCA |
GPx | CTCGGTTTCCCGTGCAATCAG | GTGCAGCCAGTAATCACCAAG |
Cu/Zn-SOD | CAGCATGGGTTCCACGTCCA | CACATTGGCCACACCGTCCT |
Adiponectin | CATGACCAGGAAACCACGACT | TGAATGCTGAGCGGTAT |
NOX4 | GAAGCCCATTTGAGGAGTCA | GGGTCCACAGCAGAAAACTC |
G6PDH | CGATGGCAGAGCAGGT | GATCTGGTCCTCACG |
GAPDH | AACTTTGGCATTGTGGAAGG | ACACATTGGGGGTAGGAACA |
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Lee, Y.-J.; Kim, K.-J.; Park, K.-J.; Yoon, B.-R.; Lim, J.-H.; Lee, O.-H. Buckwheat (Fagopyrum esculentum M.) Sprout Treated with Methyl Jasmonate (MeJA) Improved Anti-Adipogenic Activity Associated with the Oxidative Stress System in 3T3-L1 Adipocytes. Int. J. Mol. Sci. 2013, 14, 1428-1442. https://doi.org/10.3390/ijms14011428
Lee Y-J, Kim K-J, Park K-J, Yoon B-R, Lim J-H, Lee O-H. Buckwheat (Fagopyrum esculentum M.) Sprout Treated with Methyl Jasmonate (MeJA) Improved Anti-Adipogenic Activity Associated with the Oxidative Stress System in 3T3-L1 Adipocytes. International Journal of Molecular Sciences. 2013; 14(1):1428-1442. https://doi.org/10.3390/ijms14011428
Chicago/Turabian StyleLee, Young-Jun, Kui-Jin Kim, Kee-Jai Park, Bo-Ra Yoon, Jeong-Ho Lim, and Ok-Hwan Lee. 2013. "Buckwheat (Fagopyrum esculentum M.) Sprout Treated with Methyl Jasmonate (MeJA) Improved Anti-Adipogenic Activity Associated with the Oxidative Stress System in 3T3-L1 Adipocytes" International Journal of Molecular Sciences 14, no. 1: 1428-1442. https://doi.org/10.3390/ijms14011428