Int. J. Mol. Sci. 2013, 14(1), 1412-1427; doi:10.3390/ijms14011412

Shear Stress Inhibits Apoptosis of Ischemic Brain Microvascular Endothelial Cells

1,2,†email, 1,†email, 3,†email, 4,†email, 1email, 1email, 1email, 5email, 1,2email, 1email, 1email, 4email, 4email, 1,2email, 1,2email, 1email and 1,2,* email
Received: 8 August 2012; in revised form: 22 November 2012 / Accepted: 20 December 2012 / Published: 11 January 2013
(This article belongs to the Section Molecular Pathology)
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Abstract: As a therapeutic strategy for ischemic stroke, to restore or increase cerebral blood flow (CBF) is the most fundamental option. Laminar shear stress (LS), as an important force generated by CBF, mainly acts on brain microvascular endothelial cells (BMECs). In order to study whether LS was a protective factor in stroke, we investigated LS-intervented ischemic apoptosis of rat BMECs (rBMECs) through PE Annexin V/7-AAD, JC-1 and Hoechst 33258 staining to observe the membranous, mitochondrial and nuclear dysfunction. Real-time PCR and western blot were also used to test the gene and protein expressions of Tie-2, Bcl-2 and Akt, which were respectively related to maintain membranous, mitochondrial and nuclear norm. The results showed that LS could be a helpful stimulus for ischemic rBMECs survival. Simultaneously, membranous, mitochondrial and nuclear regulation played an important role in this process.
Keywords: laminar shear stress; ischemic stroke; apoptosis; brain microvascular endothelial cells
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MDPI and ACS Style

Tian, S.; Bai, Y.; Yang, L.; Wang, X.; Wu, Y.; Jia, J.; Zhu, Y.; Cheng, Y.; Zhang, P.; Wu, J.; Wang, N.; Xia, G.; Liao, H.; Zhang, Y.; Shen, X.; Yu, H.; Hu, Y. Shear Stress Inhibits Apoptosis of Ischemic Brain Microvascular Endothelial Cells. Int. J. Mol. Sci. 2013, 14, 1412-1427.

AMA Style

Tian S, Bai Y, Yang L, Wang X, Wu Y, Jia J, Zhu Y, Cheng Y, Zhang P, Wu J, Wang N, Xia G, Liao H, Zhang Y, Shen X, Yu H, Hu Y. Shear Stress Inhibits Apoptosis of Ischemic Brain Microvascular Endothelial Cells. International Journal of Molecular Sciences. 2013; 14(1):1412-1427.

Chicago/Turabian Style

Tian, Shan; Bai, Yulong; Yang, Lin; Wang, Xinggang; Wu, Yi; Jia, Jie; Zhu, Yulian; Cheng, Yong; Zhang, Pengyue; Wu, Junfa; Wang, Nianhong; Xia, Guang; Liao, Hua; Zhang, Yuling; Shen, Xiafeng; Yu, Huixian; Hu, Yongshan. 2013. "Shear Stress Inhibits Apoptosis of Ischemic Brain Microvascular Endothelial Cells." Int. J. Mol. Sci. 14, no. 1: 1412-1427.

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