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Shear Stress Inhibits Apoptosis of Ischemic Brain Microvascular Endothelial Cells
Department of Rehabilitation, Huashan Hospital, Fudan University, Shanghai 200040, China
State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200032, China
Research Center, EYE & ENT Hospital of Fudan University, Shanghai 200031, China
Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China
Department of Cardiology, Zhengzhou Center Hospital, Zhengzhou University, Zhengzhou 450000, China
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 8 August 2012; in revised form: 22 November 2012 / Accepted: 20 December 2012 / Published: 11 January 2013
Abstract: As a therapeutic strategy for ischemic stroke, to restore or increase cerebral blood flow (CBF) is the most fundamental option. Laminar shear stress (LS), as an important force generated by CBF, mainly acts on brain microvascular endothelial cells (BMECs). In order to study whether LS was a protective factor in stroke, we investigated LS-intervented ischemic apoptosis of rat BMECs (rBMECs) through PE Annexin V/7-AAD, JC-1 and Hoechst 33258 staining to observe the membranous, mitochondrial and nuclear dysfunction. Real-time PCR and western blot were also used to test the gene and protein expressions of Tie-2, Bcl-2 and Akt, which were respectively related to maintain membranous, mitochondrial and nuclear norm. The results showed that LS could be a helpful stimulus for ischemic rBMECs survival. Simultaneously, membranous, mitochondrial and nuclear regulation played an important role in this process.
Keywords: laminar shear stress; ischemic stroke; apoptosis; brain microvascular endothelial cells
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Tian, S.; Bai, Y.; Yang, L.; Wang, X.; Wu, Y.; Jia, J.; Zhu, Y.; Cheng, Y.; Zhang, P.; Wu, J.; Wang, N.; Xia, G.; Liao, H.; Zhang, Y.; Shen, X.; Yu, H.; Hu, Y. Shear Stress Inhibits Apoptosis of Ischemic Brain Microvascular Endothelial Cells. Int. J. Mol. Sci. 2013, 14, 1412-1427.
Tian S, Bai Y, Yang L, Wang X, Wu Y, Jia J, Zhu Y, Cheng Y, Zhang P, Wu J, Wang N, Xia G, Liao H, Zhang Y, Shen X, Yu H, Hu Y. Shear Stress Inhibits Apoptosis of Ischemic Brain Microvascular Endothelial Cells. International Journal of Molecular Sciences. 2013; 14(1):1412-1427.
Tian, Shan; Bai, Yulong; Yang, Lin; Wang, Xinggang; Wu, Yi; Jia, Jie; Zhu, Yulian; Cheng, Yong; Zhang, Pengyue; Wu, Junfa; Wang, Nianhong; Xia, Guang; Liao, Hua; Zhang, Yuling; Shen, Xiafeng; Yu, Huixian; Hu, Yongshan. 2013. "Shear Stress Inhibits Apoptosis of Ischemic Brain Microvascular Endothelial Cells." Int. J. Mol. Sci. 14, no. 1: 1412-1427.