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Int. J. Mol. Sci. 2013, 14(1), 1412-1427; doi:10.3390/ijms14011412
Article

Shear Stress Inhibits Apoptosis of Ischemic Brain Microvascular Endothelial Cells

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Received: 8 August 2012 / Revised: 22 November 2012 / Accepted: 20 December 2012 / Published: 11 January 2013
(This article belongs to the Section Molecular Pathology)
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Abstract

As a therapeutic strategy for ischemic stroke, to restore or increase cerebral blood flow (CBF) is the most fundamental option. Laminar shear stress (LS), as an important force generated by CBF, mainly acts on brain microvascular endothelial cells (BMECs). In order to study whether LS was a protective factor in stroke, we investigated LS-intervented ischemic apoptosis of rat BMECs (rBMECs) through PE Annexin V/7-AAD, JC-1 and Hoechst 33258 staining to observe the membranous, mitochondrial and nuclear dysfunction. Real-time PCR and western blot were also used to test the gene and protein expressions of Tie-2, Bcl-2 and Akt, which were respectively related to maintain membranous, mitochondrial and nuclear norm. The results showed that LS could be a helpful stimulus for ischemic rBMECs survival. Simultaneously, membranous, mitochondrial and nuclear regulation played an important role in this process.
Keywords: laminar shear stress; ischemic stroke; apoptosis; brain microvascular endothelial cells laminar shear stress; ischemic stroke; apoptosis; brain microvascular endothelial cells
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Tian, S.; Bai, Y.; Yang, L.; Wang, X.; Wu, Y.; Jia, J.; Zhu, Y.; Cheng, Y.; Zhang, P.; Wu, J.; Wang, N.; Xia, G.; Liao, H.; Zhang, Y.; Shen, X.; Yu, H.; Hu, Y. Shear Stress Inhibits Apoptosis of Ischemic Brain Microvascular Endothelial Cells. Int. J. Mol. Sci. 2013, 14, 1412-1427.

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