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• Kim, K
• Park, K
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• Lim, J
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• Kim, K
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• Kim, K
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Int. J. Mol. Sci. 2013, 14(1), 1428-1442; doi:10.3390/ijms14011428

Article

Buckwheat (Fagopyrum esculentum M.) Sprout Treated with Methyl Jasmonate (MeJA) Improved Anti-Adipogenic Activity Associated with the Oxidative Stress System in 3T3-L1 Adipocytes

Young-Jun Lee ^1,† , Kui-Jin Kim ^2,† , Kee-Jai Park ³ , Bo-Ra Yoon ¹ , Jeong-Ho Lim ^3,*  and Ok-Hwan Lee ^1,*

¹ Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 200-701, Korea ² Department of Cancer and Cell Biology, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA ³ Korea Food Research Institute, Gyeonggi 463-746, Korea ^† These authors contributed equally to this work.

* Authors to whom correspondence should be addressed.

Received: 9 November 2012; in revised form: 25 December 2012 / Accepted: 3 January 2013 / Published: 11 January 2013

(This article belongs to the Section Bioactives and Nutraceuticals)

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Abstract: Buckwheat sprouts contain various bioactive compounds including rutin which have a number of biological activities. We have previously shown that buckwheat sprouts (TBWE) treated with methyl jasmonate (MeJA) significantly increased the amount of phenolics and the antioxidant activity. The aim of this study was to demonstrate the effect of TBWE on anti-adipogenesis and pro-oxidant enzyme in 3T3-L1 adipocytes. We also evaluated the anti-oxidative activity of TBWE in adipocytes by using the nitroblue tetrazolium assay. Our data showed that TBWE markedly inhibited adipocyte differentiation and ROS production in 3T3-L1 cells compared with control groups. Moreover, TBWE has strongly shown the inhibition of adipogenic transcription factor as well as pro-oxidant enzymes. Together, we demonstrate that the MeJA treatment significantly increased the amount of phenolic compound, resulting in the suppression of adipogenesis and ROS production in the 3T3-L1 cells. These findings indicate that TBWE has the potential for anti-adipogenesis activity with anti-oxidative properties.

Keywords: buckwheat (Fagopyrum esculentum M.) sprouts; methyl jasmonate (MeJA); anti-adipogenic activity; 3T3-L1; ROS production; obesity

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